The sex determining region Y gene (SRY) is a critical factor responsible for the activation of Mullerian Inhibiting Substance (MIS), which initiates the regression of the Mullerian duct structures, the analgen of the uterus, fallopian tubes, and vagina. The specific regions of the MIS promoter acted upon by SRY to enhance expression are unknown, and experiments have suggested that SRY does not act alone. Recently the non-Y located genes SRY-related gene 9 (SOX 9) and the dosage sensitive sex reversal-adrenal hypoplasia congenital (DSS -AHC) critical region on the X chromosome, gene 1 (DAX 1) have been implicated in causing some forms of sex reversal. SRY is hypothesized to require an interaction with candidate factors such as SOX 9 or DAX 1 or novel factors, to activate MIS transcription. MIS promoter deletion experiments will be undertaken to define the specific regions under SRY's influence, and the yeast two hybrid screening method will be applied to test candidate factors for SRY interaction as well as to search for novel SRY interacting factors. Understanding the mechanisms by which SRY and other molecules mediates these pathways is of crucial importance to understanding sexual dimorphism.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HD008135-02
Application #
2592337
Study Section
Special Emphasis Panel (ZRG2-END (01))
Project Start
1997-07-01
Project End
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
2
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199