The long-term objectives of this work are to elucidate the molecular basis of pattern formation during development in Drosophila melanogaster. The characterization of a genetic component of the Wingless signaling pathway in the Drosophila embryonic epidermis will serve to define elements required for regulation of developmental decisions in invertebrates and, by analogy, vertebrates. The molecular cloning of arrow, a newly identified member of the Wingless pathway, will be performed and will facilitate subsequent studies designed to further identify the role arrow plays in the Wingless pathway. The generation of antibodies specific to the Arrow protein will aid in determining its cellular localization. Genetic epistasis and embryonic microinjection will provide information regarding the location, and possibly the mechanism of action, of arrow in the Wingless pathway. The implications of this work lie in the possibility that this genetic cascade will provide insights into mechanisms of vertebrate development as the Wingless pathway utilizes a novel form of signal transduction and is highly conserved evolutionarily.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HD008193-03
Application #
2673395
Study Section
Special Emphasis Panel (ZRG2-BIOL-1 (01))
Project Start
1998-09-01
Project End
Budget Start
1998-09-01
Budget End
1999-03-31
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Biology
Type
Other Domestic Higher Education
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027