Abstract

The study of HIV-1 in the genital tract of women is necessary to understand pathogenesis, vaccine design, and heterosexual and mother-to-child transmission. In addition, in this era of potent antiviral therapy, examination of HIV-1 reservoirs in the genital tract is a high priority. Some have hypothesized that viruses from the genital tract and plasma differ from each other and that these differences affect HIV-1 disease progression. To test this hypothesis, the Candidate proposes to determine complete HIV-1 RNA sequences from virus in the genital tract, specifically the cervicovaginal lavage (CVL), and compare them to contemporaneous plasma sequences from the same individual. The Candidate and associates have developed a technique based on reverse transcription and long PCR to clone full-length HIV-1 RNA genomes from infected individuals. He now proposes to use this technique to analyze samples from participants in the NIH Women's Interagency HIV Study (WIHS), a longitudinal cohort study of HIV-1 infection in women, selecting participants who represent clinically significan endpoints.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32HD008478-01
Application #
2710164
Study Section
AIDS and Related Research Study Section 2 (ARRB)
Program Officer
Nugent, Robert
Project Start
1998-12-10
Project End
Budget Start
1998-07-01
Budget End
1999-06-30
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Wadsworth Center
Department
Type
DUNS #
110521739
City
Menands
State
NY
Country
United States
Zip Code
12204

  Publications

Philpott, Sean; Burger, Harold; Tsoukas, Christos et al. (2005) Human immunodeficiency virus type 1 genomic RNA sequences in the female genital tract and blood: compartmentalization and intrapatient recombination. J Virol 79:353-63
Philpott, Sean; Burger, Harold; Tarwater, Patrick M et al. (2004) CCR2 genotype and disease progression in a treated population of HIV type 1-infected women. Clin Infect Dis 39:861-5
Philpott, Sean; Weiser, Barbara; Tarwater, Patrick et al. (2003) CC chemokine receptor 5 genotype and susceptibility to transmission of human immunodeficiency virus type 1 in women. J Infect Dis 187:569-75
Chakraborty, Rana; Gillespie, Geraldine M A; Reinis, Milan et al. (2002) HIV-1-specific CD8 T cell responses in a pediatric slow progressor infected as a premature neonate. AIDS 16:2085-7
Philpott, S; Weiser, B; Anastos, K et al. (2001) Preferential suppression of CXCR4-specific strains of HIV-1 by antiviral therapy. J Clin Invest 107:431-8