An acidic lumen pH in the epididymis and vas deferens is crucial for sperm maturation and storage. Impairment of the acidification capacity might, therefore, result in lower fertility.
The aim of this proposal is to develop a thorough understanding of the factors responsible for the regulation of acidification in the epididymis and vas deferens. The proposed studies are divided into three specific aims addressing hormonal regulation under physiological conditions, as well as pathophysiological effects of environmental toxins such as heavy metals and xenoestrogens on acidification in the male reproductive tract, by looking at the impact of these factors on the function of the vacuolar H+ATPase (PP). Our hypothesis is that the PP is regulated by various factors, having short term and long term effects. Acute and chronic regulation of net H+ secretion by aldosterone, testosterone, estrogen, and angiotensin II, will be examined. The effects of heavy metals such as cadmium, lead and mercury will also be examined. The proposed studies will use a novel and highly accessible model of epithelial acidification and a multidisciplinary approach (cell biology, immunocytochernistry, H+-selective microelectrodes), to identify and dissect the pathway of proton secretion and its regulation. These studies will provide important information related to the poorly understood process of male reproductive tract acidification, transepithelial H+ transport in general, and the effects of environmental toxins on male reproductive capacity.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HD008684-02
Application #
6402688
Study Section
Reproductive Biology Study Section (REB)
Program Officer
Rankin, Tracy L
Project Start
2001-09-05
Project End
Budget Start
2001-09-05
Budget End
2002-09-04
Support Year
2
Fiscal Year
2001
Total Cost
$45,560
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199
Pastor-Soler, NĂºria M; Fisher, Jane S; Sharpe, Richard et al. (2010) Aquaporin 9 expression in the developing rat epididymis is modulated by steroid hormones. Reproduction 139:613-21