The goal of this research project is to develop a physical map of the mouse Y chromosome. Specifically, through our proposed research we will: 1) create a collection of 200 sequence tagged sites (STSs) distributed throughout the euchromatic portion of the mouse Y chromosome, 2) use the STSs to characterize a collection of deletion-hearing Y chromosomes, and 3) use the STSs to create an ordered array of yeast and/or bacterial artificial chromosome (YAC or BAC) clones spanning the euchromatic portion of the Y chromosome. Y-specific material isolated by bivariate fluorescence activated chromosome sorting, and/or subtractive hybridization of male and female genomic DNA, will be used to create an STS source library of small insert size (500 base pairs). Three to four hundred Y-specific clones will be sequenced with the goal of generating at least 200 single copy, Y- specific, STS primer pairs. The STSs win then be used to characterize a series of deletion-bearing Y chromosomes, giving order to both the STSs and the deletion breakpoints, and thereby creating a physical map of the Y. The STSs will also be used to isolate and order a contiguous series of YAC or BAC clones spanning the Y chromosome. Together, the proposed experiments will give us a deeper understanding of the structure and evolution of the Y chromosome. Moreover, by providing a direct molecular entry point for cloning genes borne on the Y, the proposed experiments will afford new insights into the study of sex determination, male fertility, and illnesses (such as hypertension and gonadoblastoma) that show an association with certain Y-linked alleles.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HG000150-03
Application #
2635006
Study Section
Genome Study Section (GNM)
Program Officer
Graham, Bettie
Project Start
1997-12-18
Project End
Budget Start
1997-12-18
Budget End
1998-12-17
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Jackson Laboratory
Department
Type
DUNS #
042140483
City
Bar Harbor
State
ME
Country
United States
Zip Code
04609
Bergstrom, D E; Young, M; Albrecht, K H et al. (2000) Related function of mouse SOX3, SOX9, and SRY HMG domains assayed by male sex determination. Genesis 28:111-24
Bergstrom, D E; Gagnon, L H; Eicher, E M (1999) Genetic and physical mapping of the dreher locus on mouse chromosome 1. Genomics 59:291-9
Bergstrom, D E; Grieco, D A; Sonti, M M et al. (1998) The mouse Y chromosome: enrichment, sizing, and cloning by bivariate flow cytometry. Genomics 48:304-13
Bergstrom, D E; Yan, H; Sonti, M M et al. (1997) An expanded collection of mouse Y chromosome RDA clones. Mamm Genome 8:510-2