RESEARCH PROPOSAL: Scientific Merit:
The aim of this work is to establish the function of the mouse homologue of CMF-1, a novel protein characterized by the Bader laboratory as possibly playing a role in chick cardiogenesis. The cloning of the mouse homologue will be aimed at eventually knocking it out and establishing the phenotype, both in vitro and in vivo. While the scientific merit of the project as it stands now is good, the preliminary data are so scarce that a knockout approach fails to engender an outstanding level of enthusiasm. Before contemplating a knockout, a specific probe should have rapidly been generated and used for both whole mount and section-oriented in situ's. Until these data are generated, the rationale for undertaking a three year experimental plan is simply too weak.
|Pabon-Pena, L M; Goodwin, R L; Cise, L J et al. (2000) Analysis of CMF1 reveals a bone morphogenetic protein-independent component of the cardiomyogenic pathway. J Biol Chem 275:21453-9|
|Dees, E; Pabon-Pena, L M; Goodwin, R L et al. (2000) Characterization of CMF1 in avian skeletal muscle. Dev Dyn 219:169-81|
|Goodwin, R L; Pabon-Pena, L M; Foster, G C et al. (1999) The cloning and analysis of LEK1 identifies variations in the LEK/centromere protein F/mitosin gene family. J Biol Chem 274:18597-604|