The general aim of the project is to determine the function of individual CaM kinase II variants in smooth muscle regulation toward the goal of obtaining a complete understanding of the function of this family of protein kinases in smooth muscle. I have already identified six variants of CaM kinase II gamma, as cDNAs, from a ferret aorta cDNA library. Their in vitro functions differ in terms of autophosphorylation and substrate phosphorylation. This suggests that they will also function differentially in the cell. The proposed study will be undertaken with the specific aims i) To test the hypothesis that all six variants of CaM kinase II gamma identified as cDNAs from an aorta cDNA library are expressed as protein in smooth muscle tissue; 2) To test the hypothesis that different variants of CaM kinase II gamma have differential functions; 3) To test the hypothesis that multiple variants of CaM kinase II delta are also expressed and contribute to the regulation of smooth muscle. Technologies to be used include, cloning, quantitative RT-PCR, mass spec sequencing, antisense knockdown, confocal microscopy and protein kinase assays.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HL074470-02
Application #
6883943
Study Section
Special Emphasis Panel (ZRG1-F10 (20))
Program Officer
Meadows, Tawanna
Project Start
2004-04-01
Project End
2007-03-31
Budget Start
2005-04-01
Budget End
2006-03-31
Support Year
2
Fiscal Year
2005
Total Cost
$53,492
Indirect Cost
Name
Boston Biomedical Research Institute
Department
Type
DUNS #
058893371
City
Watertown
State
MA
Country
United States
Zip Code
02472
Gangopadhyay, Samudra S; Kengni, Edouard; Appel, Sarah et al. (2009) Smooth muscle archvillin is an ERK scaffolding protein. J Biol Chem 284:17607-15
Gangopadhyay, Samudra S; Gallant, Cynthia; Sundberg, Eric J et al. (2008) Regulation of Ca2+/calmodulin kinase II by a small C-terminal domain phosphatase. Biochem J 412:507-16
Marganski, William A; Gangopadhyay, Samudra S; Je, Hyun-Dong et al. (2005) Targeting of a novel Ca+2/calmodulin-dependent protein kinase II is essential for extracellular signal-regulated kinase-mediated signaling in differentiated smooth muscle cells. Circ Res 97:541-9
Gangopadhyay, Samudra S; Takizawa, Norio; Gallant, Cynthia et al. (2004) Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle. J Cell Sci 117:5043-57