? This work will focus on developing fluorescent probes for nitric oxide capable of providing temporal concentration data in biological samples. Focus will be on transition metal complexes that exhibit a positive fluorescence response upon NO binding. Our strategy will follow three interrelated paths to be carried out in parallel: (1) improvement of the best current system and rapid screening of related complexes; (2) incorporation of best current complexes and other candidates identified in (1) into biocompatible polymers for use in solid-state temporal probes; (3) apply sensors uncovered in (1) and (2) to biological samples. The ultimate goal is a set of versatile temporal probes for the bioimaging of NO in living systems. ? ?

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32HL080565-01
Application #
6884477
Study Section
Special Emphasis Panel (ZRG1-F04A (20))
Program Officer
Meadows, Tawanna
Project Start
2004-12-15
Project End
2006-12-14
Budget Start
2004-12-15
Budget End
2005-12-14
Support Year
1
Fiscal Year
2005
Total Cost
$42,068
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Smith, Rhett C; Tennyson, Andrew G; Lim, Mi Hee et al. (2005) Conjugated polymer-based fluorescence turn-on sensor for nitric oxide. Org Lett 7:3573-5