Mechanical ventilation is a treatment method for lung injury, however high tidal volumes exacerbate lung injury. The resolution of wide angle fluorescence microscopy is insufficient for the measurement of precise alveolar dimensions, thus most studies of alveolar mechanics have been carried out in fixed lungs. However, it is impossible to study dynamic alveolar micromechanics in fixed lungs. Confocal microscopy of thin (1-2 micron) sections now enables precise, dynamic tracking of individual alveolar walls in three-dimensions. This investigation aims to (i) quantify heterogeneity of alveolar wall stretch, (ii) correlate alveolar type II cell location, surfactant secretion and calcium signaling with magnitude of wall stretch and (iii) determine the effect of local disease models of non-aerated alveoli on micromechanics in adjacent alveoli.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32HL080878-01
Application #
6938870
Study Section
Special Emphasis Panel (ZRG1-F10 (20))
Program Officer
Rothgeb, Ann E
Project Start
2005-04-01
Project End
2008-03-31
Budget Start
2005-04-01
Budget End
2006-03-31
Support Year
1
Fiscal Year
2005
Total Cost
$43,976
Indirect Cost
Name
St. Luke's-Roosevelt Institute for Health Sciences
Department
Type
DUNS #
623216371
City
New York
State
NY
Country
United States
Zip Code
10019
Perlman, Carrie E; Lederer, David J; Bhattacharya, Jahar (2011) Micromechanics of alveolar edema. Am J Respir Cell Mol Biol 44:34-9
Perlman, Carrie E; Bhattacharya, Jahar (2007) Alveolar expansion imaged by optical sectioning microscopy. J Appl Physiol 103:1037-44
Lindert, Jens; Perlman, Carrie E; Parthasarathi, Kaushik et al. (2007) Chloride-dependent secretion of alveolar wall liquid determined by optical-sectioning microscopy. Am J Respir Cell Mol Biol 36:688-96