? Despite the existence of established long term risk factors, there are currently no diagnostic tools for the prediction of acute coronary syndrome. The emerging technology of protein arrays provides exciting possibilities for the attainment of diagnostic patterns or biosignatures for a variety of diseases, including acute coronary syndrome. This proposal examines two possibilities for the attachment of ligands in high density protein arrays, with the goal of developing a diagnostic tool for predicting acute coronary syndrome. More specifically, the end groups on a pH-responsive polymer will be converted to aldehydes, which are known to react with biomolecules, by generating local acid formation through the use of a photoacid generator or electrolysis of water. Initial studies will examine the attachment of two proteins associated with atherosclerotic plaque rupture, matrix metalloproteinase-14 and interferon-gamma; however, the long term goal of this project is to assemble an array of biomarkers for the development of a unique predictive biosignature for acute coronary syndrome. This technology may also be applied to the detection and diagnosis of a variety of other diseases. ? ?

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HL082138-02
Application #
7105432
Study Section
Special Emphasis Panel (ZRG1-F04A (20))
Program Officer
Meadows, Tawanna
Project Start
2005-08-01
Project End
2007-07-31
Budget Start
2006-08-01
Budget End
2007-07-31
Support Year
2
Fiscal Year
2006
Total Cost
$48,796
Indirect Cost
Name
University of California Los Angeles
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Christman, Karen L; Broyer, Rebecca M; Schopf, Eric et al. (2011) Protein nanopatterns by oxime bond formation. Langmuir 27:1415-8
Christman, Karen L; Maynard, Heather D (2010) Surface patterning for generating defined nanoscale matrices. Methods Mol Biol 660:255-63
Christman, Karen L; Schopf, Eric; Broyer, Rebecca M et al. (2009) Positioning multiple proteins at the nanoscale with electron beam cross-linked functional polymers. J Am Chem Soc 131:521-7
Christman, Karen; Vayyzquez-Dorbatt, Vimary; Schopf, Eric et al. (2008) Nanoscale Growth Factor Patterns by Immobilization on a Heparin-Mimicking Polymer. J Am Chem Soc :