The lesions of atherosclerosis represent a series of highly specific cellular and molecular responses that can be viewed as an inflammatory disease process. Experimental, pathologic, and clinical observations support a critical role for macrophages in atherogenesis. Using a homology screening strategy we identified KLF4 expression in human macrophages and found it is markedly induced in response to proinflammatory cytokines (IFN-gamma, TNF-alpha, IPS) and decreased by the anti-inflammatory growth factor, transforming growth factor (TGF)-betal. Overexpression of KLF4 in macrophage cell lines potently induces macrophage activation markers (iNOS, CD11b, MMP-12, and MCP-1). In contrast, KLF4 potently inhibits anti- inflammatory gene responses mediated by TGF-beta1 and SmadS. Furthermore, studies to date suggest that KLF4 is able to form a cooperative interaction with the NF-kB family member, p65, a critical regulator for inflammation. The studies in this proposal will (1) define the molecular basis for KLF4's ability to induce iNOS and inhibit TGF-beta1 signaling in macrophages and (2) assess the role of KLF4 on macrophage effector function and atherosclerosis in vivo. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HL086157-03
Application #
7487903
Study Section
Special Emphasis Panel (ZRG1-F10-H (20))
Program Officer
Meadows, Tawanna
Project Start
2006-09-01
Project End
2009-08-31
Budget Start
2008-09-01
Budget End
2009-08-31
Support Year
3
Fiscal Year
2008
Total Cost
$58,036
Indirect Cost
Name
Brigham and Women's Hospital
Department
Type
DUNS #
030811269
City
Boston
State
MA
Country
United States
Zip Code
02115
Cao, Zhuoxiao; Sun, Xinghui; Icli, Basak et al. (2010) Role of Kruppel-like factors in leukocyte development, function, and disease. Blood 116:4404-14
Cao, Zhuoxiao; Wara, Akm Khyrul; Icli, Basak et al. (2009) Kruppel-like factor KLF10 targets transforming growth factor-beta1 to regulate CD4(+)CD25(-) T cells and T regulatory cells. J Biol Chem 284:24914-24