Two and a half million people suffer from atrial fibrillation (AF) in the United States, costing $26 billion annually. It is currently unknown whether age-induced inflammation from atherosclerosis induces atrial remodeling and fibrosis resulting in AF, although both age and atherosclerosis are major risk factors for developing AF. Age-specific therapies for AF are currently lacking and pose significant risks in the elderly, including increased risk of bleeding and falls. The long-term goals of this research are to determine the mechanisms of how aging, atherosclerosis, and inflammation interact to enhance AF and to develop novel therapies targeting these inflammatory mechanisms. The focus of this proposal is to test the hypothesis that macrophages in the atrial myocardium or epicardial adipose tissue mediate inflammation with atherosclerosis and aging which dysregulates ion channels and leads to AF. To test this hypothesis, we will use a murine model of atherosclerosis, the low-density lipoprotein receptor knockout (Ldlr-/-) mouse, and we will pursue 2 specific aims. First, we will determine if AF inducbility is dependent on inflammation and metabolic derangement from atherosclerosis. In order to complete this aim, we will maintain young wild-type (WT) mice on a chow diet or switch them to a high fat diet (HFD) for 3 months, and we will treat young Ldlr-/- mice similarly. We will determine whether vascular inflammation in the Ldlr-/- mice fed a HFD on top of metabolic derangement in WT mice fed a HFD is required for AF. We will also assess atrial remodeling, inflammation, macrophage accumulation and phenotype, and ion channel dysfunction.
Our second aim will be to determine the role of atrial macrophages on AF inducibility during atherosclerosis and aging. Based on our preliminary data, we hypothesize that macrophages are critical for AF. Thus, we will enumerate and phenotype macrophages in the atrial myocardium in young and aged Ldlr-/- and WT mice fed a chow diet or switched to a HFD for 3 months. We will also acutely deplete macrophages via pharmacologic approaches to determine if their absence reduces AF. Again, we will assess atrial remodeling, ion channel properties, and macrophage accumulation and phenotype. The proposed research will provide novel insights into the complex interplay between aging, atherosclerosis, and AF and will generate new cellular and molecular pathways to be developed into therapies to reduce the burden of AF in older people.

Public Health Relevance

Aging and atherosclerosis are major risk factors for atrial fibrillation, which is a debilitating disease primarily affecting older people. Current therapies for atrial fibrillation have serious adverse effects such as bleeding, fainting, and falls. Our proposed research will determine how aging, atherosclerosis, and inflammation interact to enhance atrial fibrillation with the goal of developing novel drug targets to specifically treat atrial fibrillation in older people.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Postdoctoral Individual National Research Service Award (F32)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Lidman, Karin Fredriksson
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Michigan Ann Arbor
Internal Medicine/Medicine
Schools of Medicine
Ann Arbor
United States
Zip Code