The brain has the capability of becoming less vulnerable to stroke. In this proposal, the endogenous development of neuronal protection, through sublethal exposure to oxygen-glucose deprivation, will be studied using an in vitro model which has been established by the applicant. The model, coined """"""""Cerebral preconditioning in Cortical Cell Culture"""""""" (CPC) will first be explored in further detail as to the extent of protection this sublethal stress has on other neuronal injury paradigms. In the next set of experiments, further characterization of the phenomenon will be initiated to explore methods of mimicking the phenomenon pharmacologically. Finally, pre and postsynaptic alterations will be studied to determine the dependence of both for the development of CPC. Presynaptically the cause of the lowered glutamate release will be defined, while postsynaptically, glutamate receptor alterations and synthesis of specific proteins will be measured. From these results, scientists will have a better handle of the probable mechanisms involved, and studies can then turn to initiating these mechanisms for clinical use.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32NS010291-02
Application #
2546419
Study Section
Special Emphasis Panel (ZRG1-NLS-3 (01))
Program Officer
Jacobs, Tom P
Project Start
1997-09-11
Project End
Budget Start
1997-09-11
Budget End
1998-09-10
Support Year
2
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Washington University
Department
Neurology
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130