The goal of this proposal is to better understand the mechanisms underlying synaptic transmission. We will study the unc-13 gene in Caenorhabditis elegans to determine its role in regulating neurotransmitter release. First, we will examine alterations in the cellular and subcellular localization of UNC-13 in mutants with defects in neurotransmission using indirect immunofluorescence. Using antibodies which recognize UNC-13 and other proteins important for synaptic transmission, we will show the localization of UNC-13 relative to other presynaptic proteins. To further investigate how unc-13 is involved in neurotransmitter release, we will search for protein-protein interactions between UNC-13 and other proteins using both extragenic suppressor screens and the two-hybrid system in yeast. While this is basic research, it is clearly relevant to health. Proper neurotransmitter release is crucial to proper function of the nervous system, and defective neurotransmitter release has been implicated in many psychiatric and neurobiological disorders.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
2F32NS010329-03
Application #
2864083
Study Section
Special Emphasis Panel (ZRG1-MDCN-1 (01))
Program Officer
Baughman, Robert W
Project Start
1999-02-01
Project End
1999-08-09
Budget Start
1999-02-01
Budget End
1999-08-09
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Oklahoma Medical Research Foundation
Department
Type
DUNS #
937727907
City
Oklahoma City
State
OK
Country
United States
Zip Code
73104