The applicant proposes to look for and characterize a neurotensin subtype that mediates the hypothermic response to ICV or intra-PAG neurotensin without producing antinociceptive effects. After characterizing the receptor with regard to ligand specificity, PI turnover and behavioral effects, the receptor subtype of interest will be localized to a specific brain region and finally cloned.
| Tyler, B M; Douglas, C L; Fauq, A et al. (1999) In vitro binding and CNS effects of novel neurotensin agonists that cross the blood-brain barrier. Neuropharmacology 38:1027-34 |
| Tyler, B M; McCormick, D J; Hoshall, C V et al. (1998) Specific gene blockade shows that peptide nucleic acids readily enter neuronal cells in vivo. FEBS Lett 421:280-4 |
| Tyler, B M; Cusack, B; Douglas, C L et al. (1998) Evidence for additional neurotensin receptor subtypes: neurotensin analogs that distinguish between neurotensin-mediated hypothermia and antinociception. Brain Res 792:246-52 |
