Recent studies indicate that nitric oxide (NO) is involved in the pathogenesis of infarction and subsequent neurodegeneration following focal ischemia. Furthermore, expression of neuronal nitric oxide synthase (nNOS or type I NOS), the enzyme responsible for generation of NO, is upregulated during focal ischemia. To examine the mechanism of this upregulation of nNOS, I propose the following: 1) To characterize in detail the upregulation of nNOS mRNA, protein level, and catalytic activity in response to ischemia. 2) To identify regions of the nNOS promoter involved in upregulation of nNOS in response to ischemia. 3) To identify potential transcription factors involved in nNOS upregulation in response to ischemia.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32NS010635-02
Application #
2883594
Study Section
Special Emphasis Panel (ZRG1-NLS-3 (01))
Program Officer
Jacobs, Tom P
Project Start
1999-03-01
Project End
Budget Start
1999-03-01
Budget End
1999-11-05
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Neurology
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218