AA PART I(Forrn Pages 1 to6, 9) PI: MERRILL, DANIEL R Council: 10/2002 Department of Health andHuman Services , 1 F32 NS045454-01 Puuio.eamser_i:eA O D _ __'}Ntt_ Individual National Resear_l_'_'_'J_Vi_ I Dual: PJ 0 ? S 2 6 IRG: ZRG1 F01(20) L Received:04/05/2002 / .................. Is',,, r_[nctton_ indic,sled on sample. I Meeting Dates l Date Received 1. TITLE OF RESEARCH TRAINING PROPOSAL (Do not exceed 58 characters, including spaces and punctuation.) Mechanisms of Brain .Tissue Response to Im.pl.anted Devices Z. LEVELOF FELLOWSHIP . 3. PROGRAM ANNOUNCEMENTfREQUEST FOR APPLICATIONS Postdoctoral 4a. NAME OF APPLICANT (Last, first, middle initial) Merrill, Daniel R. 4d, PRESENT MAILING ADDRESS (Street, city, state, zip cede) 2413 Woodmere Drive Cleveland Heights, OH 44106 4f. OFFICE TELEPHONE NO. (Area code, 4g. HOME TELEPHONE NO. no., and ext.) (Area code and no.) (216_ 368-2378 (216_ 932,7202 U,S. CITIZEN OR U.S. NONCITIZEN NATIONAL or 5. TRAINING UNDER PROPOSED AWARD (See Lexicon) Disciplin?/_J_ _mCoatmegeoarym_am_ Engineering Form n NO I I YES (If'Yes, refer to Item 24, Page 5) 7a. DATES OF PROPOSED AWARD 7b. PROPOSED AWARD DURATION 8. DEGREE SOUGHT DURING PROPOSED AWARD From (MMIDD/YY) .Through (MM/DD/YY) (in month_) Degtee Expected Completion Dote 4b. E-MAIL drm2@po.cwru.edu 4e. PERMANENT MAILING ADDRESS 31925 SE 40th Street Fall City, WA 98024 4h. PERMANENT PHONE NO. 4i. (Area code and no.) (425_222-4720 4c. HIGHEST DEGREE(S) B.S., M.Eng., PhE (Street, city, state, zip code) FAX NUMBER (Area c_de and no.) (216] 368-4R72 '_ PERMANENT RESIDENT OF U.S. 6. PRIOR AN D/OR CURRENT NRSA SUPPORT (Jndfvidual or Institutional) 9. HUMAN 9a. If 'Yes,' Exemption No. 9b. Assurance SUBJECTS _oz Compliance r-_ No IRB Approval Date _ FulllRB or Expedited r-_ YEs Review 1la. NAME OF SPONSOR (Last, first, middle initial) Tresco, Patrick, A. Telephone Fax (801) 581-8873 E-mail Addres_ (801) 585-5151 patrick, fresco@utah, edu 11c. DEPARTMENT, SERVICE, LABORATORY, OR EQUIVALENT Department of Bioengineering 11d. MAJOR SUBDIVISION College of Engineering 131 NAME AN D TE LEPHON E NO. OF ADVISOR IF DtFFERENT FROM 1la. Telephone Name and address of institution where research training will take place if different from Item 1lb. Add ross 10. VERTEBRATE of ANIMALS 10e. If=Yes,' No. IACUC approval 10b. Animal Welfare r_NO date Assurance No. RYES !0/06/01 A3031-0] 1lb. NAME OF PROPOSED SPONSORING INSTITUTION Addr_&iversity of Utah 1471 Federal Way Salt Lake City, UT 84102 12. ENTITY IDENTIFICATION NO. 876000525 DUNSNO._ava_b_ 00909-5565 14. NAMEOFOFFICIALINBUSINESSOFFICE Vincent Bogdanski, grants officer Telephone (801) 581-3008 Fax (801) 581-3007 Title Address Office of Sponsored Projects 1471 Federal Way Salt Lake City, UT 84102 E-mail _dress v.b0g_danski@osp.utah.edu 15. APPLICANT CERTIFICATION AND ACCEPTANCE: I certify that the statements herein are true, complete, and accurate to the best of my knowledge, and I agree to comply with the Public Health Service terms anc_ conditions if an award is issued as a result of this application. I am aware that any false, fictitious, or fraudulent statements or claims may subject me to criminal, civil, or administrative penalties. I certify that I have read the National Research Servioe ,award 8entice Assurance, that I will abide by the Assurance if an award is made, and that the award will not support residency training. PHS 416-1 (Rev. 12/98) FACE PAGE AA _t_l-'Ail-It(/-orm I_a9o$ 7 tO b; ,YJ Individual NRSA Application Merrill, Daniel R. NAME (Last, first, middle initial) I6 APP(TuocbAe .cTomSEplOeteUd CbyAaTpp*l/Ocan.t--follow PHS 416-1 instructions) DER, EEl YEAR FIELD INSTITUTION MENTOR B.S. [ May [ 1983 Electrical Engineering Comell University M.Eng. I May [ 1984 Electrical Engineering Comell University Joseph Rosson Case Westem Reserve University J.Thomas Mortimer 2002 Biomedical Engineering 17. APPLICANTS TRAINING/EMPLOYMENT (Aflercollege) ACTIVITY/ BEGINNING ENDING OCCUPATION DATE (mmlyy) DATE {rnrrdyy) FIELD INSTITUTION/COMPANY SUPERVISOPdEMPLOYER Engineer 07/84 05/86 Electrical Engineering, Honeywell, Inc. Richard Knapp Underwater Acoustics Principal 05/86 07/95 Electrical Engineering, Quinton Instrument Co. Alex Bellamy Engineer Biomedical Instrumentation 18. GQALSFOR.FELLOWSHI.TpRAININGANDCAEIEER. . intend to continue research m the 6arectmn ot engineering approaches to restoring degraded function in the nervous system. My doctoral training has involved electrochemical processes occurring on stimulating electrodes. I now desire to shift my focus to tissue engineering approaches to interface and control or regeneration of nervous tissue. I believe there is substantial unexploited overlap in the fields of functional electrical stimulation of excitable tissue, including its electrochemical implications, and cellular and tissue enginzering approaches to nervous system repair. I wish to span these areas in my academic research career. My experimental weakness is in tissue engineering laboratory skills, including cell culturing and histological methods; thus I desire to collaborate with investigators who can mentor such experimental skills while benefiting from my background. The project proposed here addresses well my desired experimental training, and involves ceil culturing, animal surgery, electrophysiological recording, fixation and tissue handling, immunohistochemieal technique, and other supporting laboratory techniques. At the end of this training period I will possess a very robust and useful set of skills for which to study biomaterial interaction with the nervous system as applied to numerous approaches for repair. 19. NAME AND DEGREE(S) 20. POSITION]RANK 21. RESEARCH INTERESTS/AREAS 22. DESCRIPTION (Do not exceed SP( )NSOR Patrick A. Tresco_ PhD Associate Profe ..... Biomaterials, Mechanisms of wound healing & Tissue Eng. space provided) (]) The mechanisms underlying decreased performance of chronically implanted biomedical devices will be investigated. Microelectrode arrays will be implanted into the auditory cortex of adult rats. Electrophysiological recordings will be performed to measure electrode impedance and record evoked single u_nit activity. Animals will be sacrificed from 3 to 28 days post-implant and immunohistochemical methods applied. The adsorbed cell t3.qgeson retrieved microelectrodes will be evaluated. Changes in electrical impedance and recording activity will be correlated with tissue response. The objective is to determine how microglial ceils and astrocytic components contribute to increasing electrical impedance and loss of single unit recording with recording electrodes. (2) The impedance of microelectrodes will be characterized in vitro based on adsorbing species availability. After baseline characterization, i extracellular molecules will be added to the media including proteins and ceils (reactive microglia, astrocytes, and! fibroblasts) and characterization will continue. Cultures will be fixed at various times and examined using indirect immunohistochemistry and cell type specific markers. Changes in impedance will be correlated with adsorbing species and the extent of cell colonization. (3) A resting 3-D culture model of cortical brain tissue will be created using mixed primary ceils suspended in an extracellular matrix gel. Serum treated probes will be implanted, and the response evaluated using microscopic methods and indirect immunohistochemistry. The ability to detect sequential ;changes in rnicroglial activation will be evaluated. The long-term objective of this project is to formulate approaches r v _a' i nalitv and to better understand the wound healin_onse in brain. PHS 416-1 (Rev. 12198) Form Page 2 BB cc NAME (Last, first, middle initial) Individual NRSA Application Table of Contents ========================================Section End===========================================
| Merrill, Daniel R; Bikson, Marom; Jefferys, John G R (2005) Electrical stimulation of excitable tissue: design of efficacious and safe protocols. J Neurosci Methods 141:171-98 |
| Merrill, Daniel R; Tresco, Patrick A (2005) Impedance characterization of microarray recording electrodes in vitro. IEEE Trans Biomed Eng 52:1960-5 |
