The objective of the proposed research is to determine the effects of lone-term estrogen treatment on the structure of individual neurons in the mammalian brain. Although estrogen improves memory and reduces the risk of developing Alzheimer's disease in post-menopausal women. and improves performance on certain learning and memory tasks in rodents, its effects on individual cortical neurons are not yet understood. Recent evidence indicates that short-term estrogen treatment causes structural changes in cortical neurons. The effects of long-term estrogen treatment. however. on the dendritic structure of neurons in the brain have not been examined. The first specific aim is to test the hypothesis that long-term estrogen treatment affects the structure of neurons in the hippocampal formation of young adult and aged female rats. The hippocampal formation is crucial for certain forms of learning and memory in humans and rodents. The second specific aim is to test the hypothesis that structural changes following estrogen treatment correlate with performance on a learning and memory task known to be mediated by the hippocampal formation. To accomplish specific aim 1, young adult and aged female rats will be ovariectomized. and a subgroup of each age group will be treated with estrogen. The structure of neurons in the hippocampal formation of the ovariectomized, estrogen-treated, and control animals will be quantified and compared between groups. To accomplish specific aim 2, the learning abilities of animals in the groups under specific aim I will be tested on a task known to be mediated by the hippocampus, and the behavioral data wil l be correlated with the structural data. Results will indicate whether or not long-term estrogen treatment causes structural changes in hippocampal neurons. and whether or not such changes correlate with performance on a learning and memory task. Given the wide-spread clinical use of Estrogen Replacement Therapy and its known cognitive effects, it is imperative that we develop an understanding of the effects of long-term estrogen treatment on neurons in the mammalian brain. It will not be possible to develop equivalent, non-steroidal therapies for memory impairments in postmenopausal %women, or in aged men, until the underlying cellular mechanisms of estrogen's cognitive effects are elucidated.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Texas Health Science Center San Antonio
San Antonio
United States
Zip Code
Ye, Ruquan; Dong, Juncai; Wang, Luqing et al. (2018) Manganese deception on graphene and implications in catalysis. Carbon N Y 132:623-631
Everett, James; Collingwood, Joanna F; Tjendana-Tjhin, Vindy et al. (2018) Nanoscale synchrotron X-ray speciation of iron and calcium compounds in amyloid plaque cores from Alzheimer's disease subjects. Nanoscale 10:11782-11796
Ortega, Eduardo; Ponce, Arturo; Santiago, Ulises et al. (2017) Structural damage reduction in protected gold clusters by electron diffraction methods. Adv Struct Chem Imaging 2:12
Rodriguez, Roberto A; Chen, Liao Y; Plascencia-Villa, Germán et al. (2017) Elongation affinity, activation barrier, and stability of A?42 oligomers/fibrils in physiological saline. Biochem Biophys Res Commun 487:444-449
Mimun, L Christopher; Ajithkumar, G; Rightsell, Chris et al. (2017) Synthesis and characterization of Na(Gd0.5Lu0.5)F4: Nd3+,a core-shell free multifunctional contrast agent. J Alloys Compd 695:280-285
Raphael, Itay; Webb, Johanna; Gomez-Rivera, Francisco et al. (2017) Serum Neuroinflammatory Disease-Induced Central Nervous System Proteins Predict Clinical Onset of Experimental Autoimmune Encephalomyelitis. Front Immunol 8:812
Srinivasan, Anand; Torres, Nelson S; Leung, Kai P et al. (2017) nBioChip, a Lab-on-a-Chip Platform of Mono- and Polymicrobial Biofilms for High-Throughput Downstream Applications. mSphere 2:
Rozinek, Sarah C; Thomas, Robert J; Brancaleon, Lorenzo (2016) Biophysical characterization of the interaction of human albumin with an anionic porphyrin. Biochem Biophys Rep 7:295-302
Plascencia-Villa, Germán; Ponce, Arturo; Collingwood, Joanna F et al. (2016) High-resolution analytical imaging and electron holography of magnetite particles in amyloid cores of Alzheimer's disease. Sci Rep 6:24873
Mendoza-Cruz, Rubén; Bazán-Díaz, Lourdes; Velázquez-Salazar, J Jesús et al. (2016) Helical Growth of Ultrathin Gold-Copper Nanowires. Nano Lett 16:1568-73

Showing the most recent 10 out of 181 publications