Proposed is a cross disciplinary training and research program designed to utilize and complement the background of the candidate by building the necessary components for a successful genome research career. Under the guidance of the advisors, the training and research plans will provide the candidate with an ideal background to contribute toward the realization of the goals of the Human Genome Project. The training aspect of this award is an in-depth study of contemporary molecular genetics and enzymology through formal coursework. In addition, the candidate will rotate through the various laboratories in the Institute for Molecular Genetics at Baylor College of Medicine. During this phase, the candidate will interact with scientists to develop an understanding of current laboratory techniques. Furthermore, local research lectures and national research conferences will be attended. Three research projects will be conducted during this award. The goal of the first project is to develop a technique which can determine semiquantitatively the number of tandemly repeated triplets in a given DNA sequence. This project will be applied to the diagnosis of myotonic dystrophy. It is proposed that an increase in the length of the tandemly repeated element increases the rate of hybridization due to an increase in the number of binding sites available for nucleation. An oligonucleotide array approach will be utilized in this study, and if successful, will demonstrate that this type of an approach can be applied to sequence analysis of DNA which contains repeated elements. The second project is an oligonucleotide array approach to genome mapping, in which an array of repeat elements are used to fingerprint an unknown DNA sequence. Finally, mutation detection chips, which can also determine sequence information, will be developed. These three projects will provide feasibility studies to evaluate oligonucleotide array approaches to both genome mapping and sequence analysis.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Scientist Development Award - Research & Training (K01)
Project #
5K01HG000014-05
Application #
2655181
Study Section
Genome Research Review Committee (GRRC)
Project Start
1994-02-01
Project End
1998-08-11
Budget Start
1998-03-13
Budget End
1998-08-11
Support Year
5
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Baylor College of Medicine
Department
Genetics
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
Shumaker, J M; Metspalu, A; Caskey, C T (1996) Mutation detection by solid phase primer extension. Hum Mutat 7:346-54