? ? Diane S. Allen-Gipson, PhD, is an Instructor at the University of Nebraska Medical Center (UNMC). After her doctoral studies at Florida A&M University, Dr. Allen-Gipson completed a post-doctoral fellowshiip at UNMC and has advanced to faculty status. As an underrepresented minority, this award mechanism, Mentored Career Development Award to Promote Faculty Diversity in Biomedical Research (K01), would help Dr. Allen-Gipson advance her academic research career by providing mentoring and resources. The environment at UNMC is ideal for nurturing young scientists to academic maturity. Dr. Allen-Gipson has superior technical resources at her disposal in the new laboratory space in the state-of-the-art Durham Research Center. Likewise, intellectual resources abound ranging from academic opportunities & seminars to her mentor, Todd A. Wyatt, PhD, and co-mentor, Joseph H. Sisson, MD, and her Advisory Committee. Her research project is primed for further study. Adenosine, the metabolic breakdown product of ATP, is recognized as an important modulator of hypoxic, ischemic, and inflammatory processes critical to both tissue homeostasis and injury. Adenosine produces a wide variety of physiological effects through activation of four cell-surface receptors, A-i, AaA, A2B and A3. Through these receptors, adenosine can either protect or damage tissues depending on the receptor(s) activated. As a potent regulator of inflammation, adenosine initiates the first stage of wound-healing. If repair responses restore normal tissue architecture, function will be preserved. Our preliminary data demonstrate the multidimensional properties of adenosine in the airway, particularly in understanding receptor regulation and signaling. These findings led us to hypothesize that: Adenosine modulates airway homeostasis and wound repair. To test this hypothesis we propose the following four Specific Aims: 1. Identify the adenosine receptor(s) present on normal and wounded airway epithelium; 2. Characterize and determine the effects of adenosine on airway wound healing and modulation of wound repair processes; 3. Identify the signaling pathways that control adenosine-mediated homeostatic and wound repair processes in airway epithelium; and 4. Characterize the effects in vitro of adenosine- mediated epithelial homeostatic and wound repair processes using primary mouse epithelial cells. These proposed studies could lead to novel therapies for inflammatory airway diseases, such as COPD. (End of Abstract) ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Scientist Development Award - Research & Training (K01)
Project #
5K01HL084684-02
Application #
7292697
Study Section
Special Emphasis Panel (ZHL1-CSR-B (F2))
Program Officer
Rothgeb, Ann E
Project Start
2006-09-25
Project End
2011-08-31
Budget Start
2007-09-01
Budget End
2008-08-31
Support Year
2
Fiscal Year
2007
Total Cost
$135,240
Indirect Cost
Name
University of Nebraska Medical Center
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
168559177
City
Omaha
State
NE
Country
United States
Zip Code
68198
Tian, Zhi; Zhang, Hui; Dixon, Jendayi et al. (2017) Cigarette Smoke Impairs A2A Adenosine Receptor Mediated Wound Repair through Up-regulation of Duox-1 Expression. Sci Rep 7:44405
Traphagen, Nicole; Tian, Zhi; Allen-Gipson, Diane (2015) Chronic Ethanol Exposure: Pathogenesis of Pulmonary Disease and Dysfunction. Biomolecules 5:2840-53
Allen-Gipson, Diane S; Zimmerman, Matthew C; Zhang, Hui et al. (2013) Smoke extract impairs adenosine wound healing: implications of smoke-generated reactive oxygen species. Am J Respir Cell Mol Biol 48:665-73
Allen-Gipson, Diane S; Blackburn, Michael R; Schneider, Daniel J et al. (2011) Adenosine activation of A(2B) receptor(s) is essential for stimulated epithelial ciliary motility and clearance. Am J Physiol Lung Cell Mol Physiol 301:L171-80
Allen-Gipson, Diane S; Jarrell, Justin C; Bailey, Kristina L et al. (2009) Ethanol blocks adenosine uptake via inhibiting the nucleoside transport system in bronchial epithelial cells. Alcohol Clin Exp Res 33:791-8
Poole, Jill A; Alexis, Neil E; Parks, Conrad et al. (2008) Repetitive organic dust exposure in vitro impairs macrophage differentiation and function. J Allergy Clin Immunol 122:375-82, 382.e1-4
Allen-Gipson, Diane S; Spurzem, Karl; Kolm, Nicole et al. (2007) Adenosine promotion of cellular migration in bronchial epithelial cells is mediated by the activation of cyclic adenosine monophosphate-dependent protein kinase A. J Investig Med 55:378-85
Slager, Rebecca E; Allen-Gipson, Diane S; Sammut, Alexi et al. (2007) Hog barn dust slows airway epithelial cell migration in vitro through a PKCalpha-dependent mechanism. Am J Physiol Lung Cell Mol Physiol 293:L1469-74