This K0l (MRSDA) application outlines training and supervised research in both experimental and theoretical approaches for understanding modulatory influences on cortical network behavior. The core of this proposal involves investigation of a central hypothesis that intrinsic ensemble dynamics of cortical networks will change in response to perturbations of the system by modulatory neurotransmitters. The proposal consists of specific aims for understanding the dynamics of isolated cortical networks to be accomplished within the award as well as the development of an experimental and theoretical framework on which to base extended career goals. ? ? Theory and experiment are integrated throughout the proposed work, however the initial phase of the award is focused on learning and applying imaging and electro-physiology techniques to map network behavior in cortical slices and understand the influence of modulatory neurotransmitters. The second period of the proposed work is more heavily theoretical, and also involves a didactic period as suggested in KO1 guideline. The final phase is more oriented towards career development, with translation towards fully independent funding and research as described in the NIMH KO1 guidelines. The candidate's long-term goals are to explore the effects of other diffuse influences on cortical function such as metabolism, stress and disease, and to move studies like these towards in-vivo preparations. ? ? The candidate is well suited to leverage the proposed training towards the investigation of the problems outlined in short and long-term goals. The candidate has both a diverse experimental background, most recently focused on the cell biology of neurodegeneration, and theoretical experience with detailed biophysical models at the compartment level as well as at the level of large-scale spatiotemporal simulations. The advanced imaging techniques, the work in mammals at a systems level and the theoretical techniques outlined in the proposal are new to the candidate. Both experimental and theoretical mentors are leaders in their respective fields and are ideal for the proposed training. NYU and the Courant Institute offer a rich environment for theory and experimental interactions, and the experimental mentor offers a superbly equipped laboratory for the proposed experimental work at Columbia. Finally, the candidate proposes an advisory committee, as suggested in KO1 guidelines, for consultation on various aspects of the proposed work.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Scientist Development Award - Research & Training (K01)
Project #
5K01MH064611-06
Application #
7192410
Study Section
Integrative, Functional and Cognitive Neuroscience 8 (IFCN)
Program Officer
Churchill, James D
Project Start
2002-09-01
Project End
2008-08-31
Budget Start
2006-09-01
Budget End
2008-08-31
Support Year
6
Fiscal Year
2006
Total Cost
$108,814
Indirect Cost
Name
University of Illinois at Chicago
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
098987217
City
Chicago
State
IL
Country
United States
Zip Code
60612
Diekman, Casey O; Fall, Christopher P; Lechleiter, James D et al. (2013) Modeling the neuroprotective role of enhanced astrocyte mitochondrial metabolism during stroke. Biophys J 104:1752-63
Oster, Andrew M; Thomas, Balbir; Terman, David et al. (2011) The low conductance mitochondrial permeability transition pore confers excitability and CICR wave propagation in a computational model. J Theor Biol 273:216-31
Dixon, Angela; Takayama, Shuichi (2010) Guided corona generates wettability patterns that selectively direct cell attachment inside closed microchannels. Biomed Microdevices 12:769-75
Caicedo, Hector H; Hernandez, Maximiliano; Fall, Christopher P et al. (2010) Multiphysics simulation of a microfluidic perfusion chamber for brain slice physiology. Biomed Microdevices 12:761-7
Mohammed, Javeed Shaikh; Caicedo, Hector Hugo; Fall, Christopher P et al. (2008) Microfluidic add-on for standard electrophysiology chambers. Lab Chip 8:1048-55
Tek, Peter; Chiganos, Terry C; Mohammed, Javeed Shaikh et al. (2008) Rapid prototyping for neuroscience and neural engineering. J Neurosci Methods 172:263-9
Shaikh Mohammed, Javeed; Caicedo, Hugo; Fall, Christopher P et al. (2007) Brain slice stimulation using a microfluidic network and standard perfusion chamber. J Vis Exp :302
Kotak, V C; Sadahiro, M; Fall, C P (2007) Developmental expression of endogenous oscillations and waves in the auditory cortex involves calcium, gap junctions, and GABA. Neuroscience 146:1629-39
Fall, Christopher P; Rinzel, John (2006) An intracellular Ca2+ subsystem as a biologically plausible source of intrinsic conditional bistability in a network model of working memory. J Comput Neurosci 20:97-107
Fall, Christopher P; Wagner, John M; Loew, Leslie M et al. (2004) Cortically restricted production of IP3 leads to propagation of the fertilization Ca2+ wave along the cell surface in a model of the Xenopus egg. J Theor Biol 231:487-96