This research proposal is to establish the macaque monkey as a model for studying normal primate hematopoiesis. During years 1-3, the distribution of lymphocyte subsets within bone marrow, lymph node, spleen, and peripheral blood compartments will be compared and the maturation and turnover of specific subsets of cells will be assessed using cell cycle analysis and dual fluorescence flow cytometry. These techniques, together with assays to assess maturation of cells of the monocytic/granulocytic series will be employed to study the hematopathology associated with type D retrovirus and SIV infection of macaques. Studies during years 4 and 5 will extend the observations on mechanisms of virus induced hematopathology and begin to explore domestic species as models for hematopoiesis.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Scientist Development Award - Research & Training (K01)
Project #
5K01RR000055-04
Application #
3069152
Study Section
Animal Resources Review Committee (AR)
Project Start
1989-09-01
Project End
1994-08-31
Budget Start
1992-09-01
Budget End
1993-08-31
Support Year
4
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Harvard University
Department
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115