The long-term goals are to gain a better understanding of pain associated with the musculoskeletal system and the analgesia produced by physical therapy treatments. The award will allow more research-related time to expand current and develop new collaborative efforts. These collaborative efforts will be aimed at developing new techniques (isolated primary afferent recording and push-pull perfusion), new ideas (mechanisms of analgesia produced by joint mobilization) and expanding current ideas (TENS, microdialysis, muscle hyperalgesia). Interactions with scientists from multiple basic science (Anatomy, Neurosciences, Pharmacology, Chemistry, Molecular Biology) and clinical disciplines (Physical Therapy, Internal Medicine, Anesthesia, Pathology, Chiropractic) provide an interdisciplinary perspective to the examination of musculoskeletal pain and physical therapy pain treatments. Group journal clubs and laboratory meetings are held weekly with several laboratories (Gebhart, Brennan, Hammond, Proudfit, Sluka) investigating pain. The research proposal in this application is designed to characterize a newly developed animal model of chronic pain induced by two unilateral injections of low pH saline into the gastrocnemius muscle. In the work proposed they hypothesize that the development of the long lasting bilateral hyperalgesia is dependent initially on activation of acid sensing ion channels (ASIC) from the site of injection. Activation of acid sensing ion channels results in long lasting, widespread hyperalgesia that is sustained by activation of central mechanisms in the spinal cord. These proposed studies are intended to help in the understanding and thus potential treatment of chronic muscle pain including such conditions as fibromyalgia, myofascial pain and low back pain.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Research Scientist Development Award - Research (K02)
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Study Section
Arthritis and Musculoskeletal and Skin Diseases Special Grants Review Committee (AMS)
Program Officer
Panagis, James S
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University of Iowa
Other Health Professions
Schools of Medicine
Iowa City
United States
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Tillu, D V; Gebhart, G F; Sluka, K A (2008) Descending facilitatory pathways from the RVM initiate and maintain bilateral hyperalgesia after muscle insult. Pain 136:331-9
Yokoyama, Takeshi; Maeda, Yumi; Audette, Katherine M et al. (2007) Pregabalin reduces muscle and cutaneous hyperalgesia in two models of chronic muscle pain in rats. J Pain 8:422-9
Hingne, Priyanka M; Sluka, Kathleen A (2007) Differences in waveform characteristics have no effect on the anti-hyperalgesia produced by transcutaneous electrical nerve stimulation (TENS) in rats with joint inflammation. J Pain 8:251-5
Yokoyama, Takeshi; Lisi, Tammy L; Moore, Steven A et al. (2007) Muscle fatigue increases the probability of developing hyperalgesia in mice. J Pain 8:692-9
Sluka, Kathleen A; Radhakrishnan, Rajan; Benson, Christopher J et al. (2007) ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation. Pain 129:102-12
Sluka, K A; Audette, K M (2006) Activation of protein kinase C in the spinal cord produces mechanical hyperalgesia by activating glutamate receptors, but does not mediate chronic muscle-induced hyperalgesia. Mol Pain 2:13
Lisi, Tammy L; Sluka, Kathleen A (2006) A new electrochemical HPLC method for analysis of enkephalins and endomorphins. J Neurosci Methods 150:74-9
Radhakrishnan, Rajan; Sluka, Kathleen A (2005) Deep tissue afferents, but not cutaneous afferents, mediate transcutaneous electrical nerve stimulation-Induced antihyperalgesia. J Pain 6:673-80
Skyba, D A; Lisi, T L; Sluka, K A (2005) Excitatory amino acid concentrations increase in the spinal cord dorsal horn after repeated intramuscular injection of acidic saline. Pain 119:142-9
Skyba, David A; Radhakrishnan, Rajan; Sluka, Kathleen A (2005) Characterization of a method for measuring primary hyperalgesia of deep somatic tissue. J Pain 6:41-7

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