Abstract

The pathogenesis of AIDS will be studied using a molecularly cloned, acutely pathogenic, simian immunodeficiency virus, SIVsmmPBj14. A collaboration with a highly-regarded primatologist at the University of Alabama at Birmingham (Dr. P. Fultz) has been established and experiments are proposed to determine the pathobiologic significance of mutations in cis-acting sequences which regulates viral transcription. Other studies focus on improving gene therapeutic approaches to HIV treatment. These experiments involve the characterization and analysis of cis-acting DNA elements which regulate the DNA replication of a ubiquitous human virus, human herpesvirus 6 (HHV-6). Ultimately, these studies are expected to result in the development of gene delivery vectors or artificial mini- chromosomes"""""""" based on this T-lymphotropic herpesvirus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Modified Research Career Development Award (K04)
Project #
5K04AI001240-04
Application #
2429341
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1994-06-01
Project End
1999-05-31
Budget Start
1997-06-01
Budget End
1998-05-31
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Rochester
Department
Microbiology/Immun/Virology
Type
Schools of Dentistry
DUNS #
208469486
City
Rochester
State
NY
Country
United States
Zip Code
14627

  Publications

Skrincosky, D; Willis, R A; Hocknell, P K et al. (2001) Epitope mapping of human herpesvirus-7 gp65 using monoclonal antibodies. Arch Virol 146:1705-22
Dewhurst, S L; da Cruz, R L; Whetter, L (2000) Pathogenesis and treatment of HIV-1 infection: recent developments (Y2K update). Front Biosci 5:D30-49
Skrincosky, D; Hocknell, P; Whetter, L et al. (2000) Identification and analysis of a novel heparin-binding glycoprotein encoded by human herpesvirus 7. J Virol 74:4530-40
Norton, R A; Caserta, M T; Hall, C B et al. (1999) Detection of human herpesvirus 6 by reverse transcription-PCR. J Clin Microbiol 37:3672-5
Dominguez, G; Dambaugh, T R; Stamey, F R et al. (1999) Human herpesvirus 6B genome sequence: coding content and comparison with human herpesvirus 6A. J Virol 73:8040-52
Hodge, S; de Rosayro, J; Glenn, A et al. (1999) Postinoculation PMPA treatment, but not preinoculation immunomodulatory therapy, protects against development of acute disease induced by the unique simian immunodeficiency virus SIVsmmPBj. J Virol 73:8630-9
Saucier, M; Hodge, S; Dewhurst, S et al. (1998) The tyrosine-17 residue of Nef in SIVsmmPBj14 is required for acute pathogenesis and contributes to replication in macrophages. Virology 244:261-72
Deng, S X; Panahian, N; James, H et al. (1998) Luciferase: a sensitive and quantitative probe for blood-brain barrier disruption. J Neurosci Methods 83:159-64
Deng, H; Dewhurst, S (1998) Functional identification and analysis of cis-acting sequences which mediate genome cleavage and packaging in human herpesvirus 6. J Virol 72:320-9
Hodge, S; Novembre, F J; Whetter, L et al. (1998) Induction of fas ligand expression by an acutely lethal simian immunodeficiency virus, SIVsmmPBj14. Virology 252:354-63

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