The overall research objectives are to determine the role of hemodynamic factors in the physiology of the pulp and gingiva in health and disease. Macrocirclulatory methods will be applied with the aim of characterizing hemodynamics of the normal and pathologically altered pulp and gingiva at the organ level, and microcirculatory functions at the local level. Macrocirculatory measurements of blood flow will be made by both the Xe-133 Washout and the Microsphere methods in dogs. Microcirculatory studies will be conducted in the rodent pulp and in the canine gingiva with microscopic determination of the following parameters in single unbranched microvessels in the microvascular network. These include the red cell velocity by the """"""""two slit"""""""" photometric technique, the distribution of volumetric flow and vascular pattern. Capillary permeability of the pulp and gingiva following various interventions will be assessed by fluorescence vital microscope. Corrosion resin vascular casts of the pathologically altered pulp and gingiva will be examined under a S.E.M. The effects of electrical stimulation of the cervical sympathetic nerve, various humoral interventions, changes of rheological parameters and oxygen transport, and experimentally induced hypotension on macro- and microcirculatory hemodynamics will be determined. The micro-/macrocirculatory findings under various perturbations will be correlated. Furthermore, the macrocirculatory methods will allow an assessment of hemodynamic contributions to the pulp-gingiva (i.e., endoperio) relationship in health and sisease states. This project, by using well established methods of inducing inflammation in the pulp and gingiva and newly developed macro- and microcirculatory techniques will allow the systematic evaluation of the role of hemodynamic factors in the physiology of the pulp and gingiva in health and disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Modified Research Career Development Award (K04)
Project #
5K04DE000121-03
Application #
3072098
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1983-07-01
Project End
1988-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027
Kim, S; Dorscher-Kim, J E; Liu, M et al. (1992) Functional alterations in pulpal microcirculation in response to various dental procedures and materials. Proc Finn Dent Soc 88 Suppl 1:65-71
Kim, S; Liu, M; Simchon, S et al. (1992) Effects of selected inflammatory mediators on blood flow and vascular permeability in the dental pulp. Proc Finn Dent Soc 88 Suppl 1:387-92
Pashley, E L; Horner, J A; Liu, M et al. (1992) Effects of CO2 laser energy on dentin permeability. J Endod 18:257-62
Markowitz, K; Bilotto, G; Kim, S (1991) Decreasing intradental nerve activity in the cat with potassium and divalent cations. Arch Oral Biol 36:1-7
Kim, S (1990) Neurovascular interactions in the dental pulp in health and inflammation. J Endod 16:48-53
Kim, S; Liu, M; Markowitz, K et al. (1990) Comparison of pulpal blood flow in dog canine teeth determined by the laser Doppler and the 133xenon washout methods. Arch Oral Biol 35:411-3
Kim, S; Dorscher-Kim, J E; Lipowsky, H H (1989) Quantitative assessment of microcirculation in the rat dental pulp in response to alpha- and beta-adrenergic agonists. Arch Oral Biol 34:707-12
Kim, S; Dorscher-Kim, J; Liu, M T et al. (1988) Biphasic pulp blood-flow response to substance P in the dog as measured with a radiolabelled, microsphere injection method. Arch Oral Biol 33:305-9
Bilotto, G; Markowitz, K; Kim, S (1988) Effects of ionic and non-ionic solutions on intradental nerve activity in the cat. Pain 32:231-8
Kogushi, M; Nakamura, S; Kishi, Y et al. (1988) A study of leukocyte extravasation in early inflammatory changes in the pulp. J Endod 14:475-81

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