Dr. Kukuruzinska's research focuses on the regulation of protein N- glycosylation. Her work has identified the first gene, ALG7, in the dolichol pathway of protein N-glycosylation as one of the determinants of the N-glycosylation characteristics of proteins and cells. Recently, she has shown that ALG7 is an early growth-response gene and that it is progressively downregulated with the normal development of the submandibular gland (SMG). The proposed research plan is to study ALG7 expression in differentiating acinar and ductal cells during the hamster SMG development, with the emphasis on the ALG7 transcript diversity and the activity of the protein product, GPT.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Modified Research Career Development Award (K04)
Project #
5K04DE000362-04
Application #
2518101
Study Section
Oral Biology and Medicine Subcommittee 1 (OBM)
Project Start
1994-09-01
Project End
1999-08-31
Budget Start
1997-09-01
Budget End
1998-08-31
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Boston University
Department
Dentistry
Type
Schools of Dentistry
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118
Fernandes, R P; Cotanche, D A; Lennon-Hopkins, K et al. (1999) Differential expression of proliferative, cytoskeletal, and adhesive proteins during postnatal development of the hamster submandibular gland. Histochem Cell Biol 111:153-62
Lennon, K; Bird, A; Kukuruzinska, M A (1997) Deregulation of the first N-glycosylation gene, ALG7, perturbs the expression of G1 cyclins and cell cycle arrest in Saccharomyces cerevisiae. Biochem Biophys Res Commun 237:562-5
Lennon, K; Bird, A; Chen, Y F et al. (1997) The dual role of mRNA half-lives in the expression of the yeast ALG7 gene. Mol Cell Biochem 169:95-106
Mota, O M; Huang, G T; Kukuruzinska, M A (1994) Developmental regulation and tissue-specific expression of hamster dolichol-P-dependent N-acetylglucosamine-1-P transferase (GPT). Biochem Biophys Res Commun 204:284-91