The research proposal focuses on our finding of a growth hormone releasing hormone-like substance (GHRH-LI) in testis and placenta. We have identified GHRH-LI in rat testis and placenta by two distinct, complementary methods: by an enzyme-linked immunosorbent assay (ELISA), we detected GHRH-LI peptide and by nucleic acid hybridization techniques, we detected GHRH-LI mRNA. Northern gel analyses revealed that the GHRH-LI mRNA species in testis is of significantly larger size than the GHRH message in placenta and hypothalamus. Recently, Western gel analyses also demonstrated that testicular GHRH-LI peptide is larger than peptide detected from either hypothalamus or placenta. During the next five years, we plan to further characterize GHRH-LI in testis and placenta by sequencing and localizing both the peptide and the mRNA. Furthermore, through the studies proposed in this application, we hope to define a physiologic function for GHRH-LI in both placenta and testis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Modified Research Career Development Award (K04)
Project #
5K04DK002042-05
Application #
2133710
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1991-08-01
Project End
1996-07-31
Budget Start
1995-08-01
Budget End
1996-07-31
Support Year
5
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Indiana University-Purdue University at Indianapolis
Department
Pediatrics
Type
Schools of Medicine
DUNS #
005436803
City
Indianapolis
State
IN
Country
United States
Zip Code
46202
Roth, S; Abernathy, M P; Lee, W H et al. (1996) Insulin-like growth factors I and II peptide and messenger RNA levels in macrosomic infants of diabetic pregnancies. J Soc Gynecol Investig 3:78-84
Srivastava, C H; Monts, B S; Rothrock, J K et al. (1995) Presence of a spermatogenic-specific promoter in the rat growth hormone-releasing hormone gene. Endocrinology 136:1502-8
Srivastava, C H; Breyer, P R; Rothrock, J K et al. (1993) A new target for growth hormone releasing-hormone action in rat: the Sertoli cell. Endocrinology 133:1478-81
Srivastava, C H; Collard, M W; Rothrock, J K et al. (1993) Germ cell localization of a testicular growth hormone-releasing hormone-like factor. Endocrinology 133:83-9