Three groups of experiments are proposed to (1) characterize the human infant's adrenocortical and behavioral responses to stressors and their interrelationships during the neonatal period and later during infancy; (2) examine individual differences in reactivity of the adrenocortical system with regard to social-emotional development; and (3) examine the dynamics of plasma (total) saliva (free, unbound) cortisol and their relations to behavioral responses to stressors and determine the effects of substituting saliva cortisol measures for plasma cortisol measures in research on children. Male and female infants (birth to 12 mos) will serve as subjects. In the experiments with newborns, ecologically relevant stressors such as circumcision, Guthrie blood-tests, weighing and measuring, and discharge examinations will be studied. behavioral state will be examined using a 30-sec coding interval and the Brazelton Neonatal Behavioral Assessment Scale will be used to measure the organization of the newborn's behavior. Activity of the pituitary-adrenocortical system will be examined using measures of plasma and saliva cortisol. Plasma samples will be obtained via heel-stick. In older infants, behavioral and adrenocortical responses to psychosocial stressors such as strangers and separation will be examined. In addition, factors mediating the infant's biobehavioral response to stressors will be explored, including characteristics of mother-infant interaction, maternal perceptions of infant temperament, and maternal support systems. Finally, behavioral correlates of plasma and saliva cortisol indices of adrenocortical activity will be examined in a population of children with phenylketonuria (PKU) during regularly scheduled clinic visits. The proposed experiments will provide basic data in the area of psychobiology as such information pertains to child development and pediatric behavioral medicine. The long-term goals are to gain a better understanding of stress and coping in human infants.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Modified Research Career Development Award (K04)
Project #
5K04HD000712-04
Application #
3073334
Study Section
Human Development and Aging Subcommittee 1 (HUD)
Project Start
1986-04-01
Project End
1991-03-31
Budget Start
1989-04-01
Budget End
1990-03-31
Support Year
4
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Type
Schools of Education
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Dobi, A; Palkovits, M; Palkovits, C G et al. (2000) Septamer element-binding proteins in neuronal and glial differentiation. J Neurosci 20:1073-84
Agoston, D V; Dobi, A (2000) Complexity of transcriptional control in neuropeptide gene expression; enkephalin gene regulation during neurodevelopment. Biochem Soc Trans 28:446-51
v Agoston, D; Santha, E; Shieh, G et al. (1998) Isolation and structural and genetic analysis of the mouse enkephalin gene and its d(AC/TG)n repeats. DNA Seq 9:217-26
Gunnar, M R; Porter, F L; Wolf, C M et al. (1995) Neonatal stress reactivity: predictions to later emotional temperament. Child Dev 66:1-13
Gunnar, M R (1992) Reactivity of the hypothalamic-pituitary-adrenocortical system to stressors in normal infants and children. Pediatrics 90:491-7
Gunnar, M R; Larson, M C; Hertsgaard, L et al. (1992) The stressfulness of separation among nine-month-old infants: effects of social context variables and infant temperament. Child Dev 63:290-303
Larson, M C; Gunnar, M R; Hertsgaard, L (1991) The effects of morning naps, car trips, and maternal separation on adrenocortical activity in human infants. Child Dev 62:362-72
Gunnar, M R; Hertsgaard, L; Larson, M et al. (1991) Cortisol and behavioral responses to repeated stressors in the human newborn. Dev Psychobiol 24:487-505
Gunnar, M R (1989) Studies of the human infant's adrenocortical response to potentially stressful events. New Dir Child Dev :3-18
Gunnar, M R; Connors, J; Isensee, J (1989) Lack of stability in neonatal adrenocortical reactivity because of rapid habituation of the adrenocortical response. Dev Psychobiol 22:221-33

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