Dr. Roess' research has involved the study of luteinizing hormone/chorionic gonadotropin (LH/CG) receptor organization and how this organization modulates receptor function. Her long-term goal is to better understand the role of LH/CG receptor structure in regulation of fertility and how gonadotropins may modulate LH/CG receptor functional events including G-protein interactions, receptor internalization and cAMP activation. During the award period she will examine whether gonadotropin binding causes size changes in functional LH receptor complexes as well as receptor-receptor aggregation on the membrane using biophysical techniques. These studies will use human embryonic kidney cells expressing LH/CG receptors with various C-terminal truncations that affect cAMP activation and receptor internalization. The effects of deglycosylated hCG and native hCG binding on LH/CG receptor association within the membrane will be evaluated on MA-10 cells where binding of deglycosylated hCG to the LH/CG receptor slows receptor down regulation and internalization. Then the association of non-receptor proteins including G-protein subunits and MHC class I antigen with LH/CG receptors will be explored by a photoproximity labeling technique that permits identification and isolation of proteins physically proximal to a known membrane site.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Modified Research Career Development Award (K04)
Project #
5K04HD001067-03
Application #
2402963
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1995-06-01
Project End
2000-05-31
Budget Start
1997-06-01
Budget End
1998-05-31
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Colorado State University-Fort Collins
Department
Physiology
Type
Schools of Veterinary Medicine
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523