The overall goal of the proposed research is to investigate specific mechanisms of transcription and regulation of transcription in eukaryotes through the characterization of trans-acting transcription factors in yeast. The SPT13 and SPT14 genes of Saccharomyces cerevisiae were identified by mutations that suppress Ty insertion mutations that inhibit adjacent gene expression. Based on both genetic and molecular experiments, SPT13 and SPT14 are candidates to act via the enhancer-like sequence in Ty (transposable) elements. The primary focus of the experiments described is the characterization of SPT13 and SPT14 in order to elucidate their mechanisms of action. In addition, the development of new genetic strategies for the direct and specific identification of genes encoding enhancer-binding factors are proposed.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Modified Research Career Development Award (K04)
Project #
5K04HG000003-04
Application #
2208265
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1990-09-28
Project End
1996-03-31
Budget Start
1994-04-12
Budget End
1995-03-31
Support Year
4
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Iowa
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242