This proposal outlines a program focused on using state-of-the-art experimental methods to address important biological problems. The immediate goal of this RCDA proposal is the development of a new high speed DNA sequencer. The realization of this instrument will allow DNA sequencing to be performed at a rate approaching one million bases per day - a rate three orders of magnitude faster than currently available techniques. To achieve this objective current DNA sequencing strategies will be combined with three new technologies to produce a low-cost, easily automated sequencing method. Laser vaporization is used to transfer tagged strands of DNA into the gas phase. Once in the gas phase resonance- enhanced, multiphoton ionization is used to place precisely one unit of positive charge on each strand and then time-of-flight mass analysis performed. The automation of the technology proposed would result in an extremely sensitive and cost effective DNA sequencing method requiring little manual labor.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Modified Research Career Development Award (K04)
Project #
5K04HG000006-02
Application #
3073560
Study Section
Genome Study Section (GNM)
Project Start
1992-07-08
Project End
1997-06-30
Budget Start
1993-07-01
Budget End
1994-06-30
Support Year
2
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Wayne State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48202