This grant proposes to expand previous investigations into protein antigens regulated by the autosomal dominant In(Lu) gene and to clone the gene(s) encoding one of these proteins. The In(Lu) gene is a unique inhibitor of expression of many genetically and biochemically unrelated blood group antigens. Such studies are anticipated to lead to further elucidation of the possible mechanism(s) whereby their expression is affected by the In(Lu) gene. The candidate has purified one protein (p80) regulated by In(Lu) and will partially sequence this protein in order to synthesize oligonucleotides with which to identify the gene. In addition, a second protein (p85), bearing Lutheran antigens, will also be purified. Receipt of an RCDA will greatly enhance this candidate's ability to perform research in her chosen field (biochemistry and regulation of expression of erythrocyte antigens) by allowing her to enter into a new field (molecular biology) with the guidance of more senior faculty and apply the acquired techniques to the area to which she has already contributed. Duke University Medical Center is able to provide professional personnel with expertise in both protein biochemistry and molecular biology with which the candidate plans to collaborate. The institution expects the candidate will use the period funded by this award to devote herself to these research efforts; this proposal will allow her to expand both her research efforts and , especially, her areas of technical expertise, in order to continue to contribute to the field of erythrocyte antigens.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Modified Research Career Development Award (K04)
Project #
5K04HL002233-02
Application #
3074391
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1988-12-01
Project End
1993-11-30
Budget Start
1989-12-01
Budget End
1990-11-30
Support Year
2
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Duke University
Department
Type
Schools of Medicine
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705
Telen, M J (1995) Blood group antigens on complement receptor/regulatory proteins. Transfus Med Rev 9:20-8
Rao, N; Udani, M; Nelson, J et al. (1995) Investigations using a novel monoclonal antibody to the glycosylphosphatidylinositol-anchored protein that carries Gregory, Holley, and Dombrock blood group antigens. Transfusion 35:459-64
Mudad, R; Rao, N; Angelisova, P et al. (1995) Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion 35:566-70
Telen, M J (1995) Glycosyl phosphatidylinositol-linked blood group antigens and paroxysmal nocturnal hemoglobinuria. Transfus Clin Biol 2:277-90
Washington, K; Gottfried, M R; Telen, M J (1994) Tissue culture of epithelium derived from Barrett's oesophagus. Gut 35:879-83
Washington, K; Gottfried, M R; Telen, M J (1994) Expression of the cell adhesion molecule CD44 in gastric adenocarcinomas. Hum Pathol 25:1043-9
Telen, M J; Rao, N (1994) Recent advances in immunohematology. Curr Opin Hematol 1:143-50
Rao, N; Whitsett, C F; Oxendine, S M et al. (1993) Human erythrocyte acetylcholinesterase bears the Yta blood group antigen and is reduced or absent in the Yt(a-b-) phenotype. Blood 81:815-9
Bobolis, K A; Moulds, J J; Telen, M J (1992) Isolation of the JMH antigen on a novel phosphatidylinositol-linked human membrane protein. Blood 79:1574-81
Telen, M J; Le Van Kim, C; Guizzo, M L et al. (1991) Erythrocyte Webb-type glycophorin C variant lacks N-glycosylation due to an asparagine to serine substitution. Am J Hematol 37:51-2

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