The applicant is an assistant professor of Gyn/Ob, Pediatrics, and Pharmacology with considerable experience in the biology of the pulmonary surfactant system and in surfactant replacement therapy. The applicant's long term career objective is to develop a multidisciplinary research program which will allow him to combine interests in basic pulmonary cell biology with experimental therapeutic approaches to the treatment of acute lung disease. The candidate's more immediate goals are to expand the current cell biology expertise of his laboratory, and to gain more hands-on experience in the area of molecular biology. The RCDA will allow the applicant to decrease his current teaching and administrative responsibilities in order to spend full time in the laboratory and achieve these goals. The applicant's Institution is supportive of his goals and agree with the candidate's perception of how to achieve them. There are a number of NIH-funded senior faculty at the State University of New York with interests in pulmonary research who collaborate with the applicant and have formed a Cardio-Pulmonary Research Group to facilitate communication. In addition, the University is proceeding with a new research building, slated for completion by 1992-93, which will provide extensive space for groups of investigators with common interest. Finally, the applicant has extensive collaborations with the University of Rochester and has been invited to spend time in the laboratory of Dr. Stuart Horowitz there in order to gain training in molecular biology. The research proposed for this award period involves studies of the cell biological mechanisms responsible for surfactant-related changes which occur in oxygen toxicity, and determination of the efficacy of therapeutic interventions directed at the cellular level. The experiments use state of the art techniques, including laser flow cytometry, to study the effects of in vivo oxygen exposure on type II pneumocyte metabolic function. These studies will then be combined with experiments to determine the efficacy of cell directed anti-oxidant therapy.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Modified Research Career Development Award (K04)
Project #
5K04HL002629-03
Application #
3074538
Study Section
Toxicology Subcommittee 2 (TOX)
Project Start
1991-08-01
Project End
1996-06-30
Budget Start
1993-07-01
Budget End
1994-06-30
Support Year
3
Fiscal Year
1993
Total Cost
Indirect Cost
Name
State University of New York at Buffalo
Department
Type
Schools of Medicine
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260
Holm, B A; Wang, Z; Egan, E A et al. (1996) Content of dipalmitoyl phosphatidylcholine in lung surfactant: ramifications for surface activity. Pediatr Res 39:805-11
Cummings, J J; Holm, B A; Nickerson, P A et al. (1995) Pre- versus post-ventilatory surfactant treatment in surfactant-deficient preterm lambs. Reprod Fertil Dev 7:1333-8
Hudak, B B; Tufariello, J; Sokolowski, J et al. (1995) Inhibition of poly(ADP-ribose) polymerase preserves surfactant synthesis after hydrogen peroxide exposure. Am J Physiol 269:L59-64
Novotny, W E; Hudak, B B; Matalon, S et al. (1995) Hyperoxic lung injury reduces exogenous surfactant clearance in vivo. Am J Respir Crit Care Med 151:1843-7
Holm, B A; Waring, A J (1993) Designer surfactants. The next generation in surfactant replacement. Clin Perinatol 20:813-29
Enhorning, G; Holm, B A (1993) Disruption of pulmonary surfactant's ability to maintain openness of a narrow tube. J Appl Physiol 74:2922-7
Cummings, J J; Holm, B A; Hudak, M L et al. (1992) A controlled clinical comparison of four different surfactant preparations in surfactant-deficient preterm lambs. Am Rev Respir Dis 145:999-1004
Enhorning, G; Shumel, B; Keicher, L et al. (1992) Phospholipases introduced into the hypophase affect the surfactant film outlining a bubble. J Appl Physiol 73:941-5