Abstract

This proposal seeks support for the applicant for a K05 level Research Scientist Award. The application, if funded, would serve to reduce the applicant's teaching and administrative assignments to a minimal level and would increase the time available to the applicant to pursue research. Most of the research that will be carried out during the tenure of this grant will use genetic strategies (classical genetic methods, molecular methods and null mutants/transgenics) to help identify nicotinic receptor subtypes that modulate specific behavioral and neurochemical responses to nicotine. A major goal is to identify mechanisms that explain genetically-based variability in initial sensitivity to nicotine and in the development of tolerance to nicotine. These studies will focus on identifying naturally-occurring polymorphisms in neuronal nicotinic receptor genes in the mouse and on assessing how/whether these polymorphisms alter behavioral and neurochemical responses to nicotine. These studies will be augmented by studies utilizing mice that have had specific nicotinic receptor genes deleted (null mutants). Hopefully, these studies will identify potential mechanisms that would explain how genetic factors influence tobacco use by humans.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Scientist Award (K05)
Project #
5K05DA000197-09
Application #
6378246
Study Section
Special Emphasis Panel (ZDA1-MXS-M (01))
Program Officer
Rutter, Joni
Project Start
1993-09-01
Project End
2003-08-31
Budget Start
2001-09-01
Budget End
2002-08-31
Support Year
9
Fiscal Year
2001
Total Cost
$119,990
Indirect Cost

  Institution

Name
University of Colorado at Boulder
Department
Genetics
Type
Other Domestic Higher Education
DUNS #
City
Boulder
State
CO
Country
United States
Zip Code
80309

  Publications

Piper, Megan E; Cook, Jessica W; Schlam, Tanya R et al. (2010) Gender, race, and education differences in abstinence rates among participants in two randomized smoking cessation trials. Nicotine Tob Res 12:647-57
Japuntich, Sandra J; Piper, Megan E; Schlam, Tanya R et al. (2009) Do smokers know what we're talking about? The construct validity of nicotine dependence questionnaire measures. Psychol Assess 21:595-607
Baker, Timothy B; Weiss, Robert B; Bolt, Daniel et al. (2009) Human neuronal acetylcholine receptor A5-A3-B4 haplotypes are associated with multiple nicotine dependence phenotypes. Nicotine Tob Res 11:785-96
Piper, Megan E; McCarthy, Danielle E; Bolt, Daniel M et al. (2008) Assessing dimensions of nicotine dependence: an evaluation of the Nicotine Dependence Syndrome Scale (NDSS) and the Wisconsin Inventory of Smoking Dependence Motives (WISDM). Nicotine Tob Res 10:1009-20
Piper, Megan E; Federman, E Belle; McCarthy, Danielle E et al. (2007) Efficacy of bupropion alone and in combination with nicotine gum. Nicotine Tob Res 9:947-54
Transdisciplinary Tobacco Use Research Center (TTURC) Tobacco Dependence; Baker, Timothy B; Piper, Megan E et al. (2007) Time to first cigarette in the morning as an index of ability to quit smoking: implications for nicotine dependence. Nicotine Tob Res 9 Suppl 4:S555-70
Whiteaker, P; Davies, A R; Marks, M J et al. (1999) An autoradiographic study of the distribution of binding sites for the novel alpha7-selective nicotinic radioligand [3H]-methyllycaconitine in the mouse brain. Eur J Neurosci 11:2689-96
Flint, J; Corley, R; DeFries, J C et al. (1995) A simple genetic basis for a complex psychological trait in laboratory mice. Science 269:1432-5
Marks, M J; Grady, S R; Yang, J M et al. (1994) Desensitization of nicotine-stimulated 86Rb+ efflux from mouse brain synaptosomes. J Neurochem 63:2125-35
Collins, A C; Luo, Y; Selvaag, S et al. (1994) Sensitivity to nicotine and brain nicotinic receptors are altered by chronic nicotine and mecamylamine infusion. J Pharmacol Exp Ther 271:125-33

Showing the most recent 10 out of 11 publications