The candidate obtained his MD and PhD in Immunology from New York University, with Dr. Jeanette Thorbecke. He then moved to the Children's Hospital, Boston, for residency in Pediatrics and a fellowship in Allergy/Immunology with Dr. Raif S. Geha. In 1986, he moved to Stanford University, as an Assistant Professor of Pediatrics. The academic environment at Stanford is outstanding in basic science immunology, where the candidate is in the Pediatric Allergy/Pulmonary Division. He is a member of the Immunology Program Faculty and has an Immunology Program graduate student in the lab. In addition, the candidate participates in the MacArthur Program Project in the Molecular Biology of Parasites at Stanford, which supports a postdoc in the lab. The career goals of the candidate are to establish his lab as a leader in the analysis of CD4+ T cell heterogeneity, especially as it relates to regulation of allergic diseases. In addition, he wishes to further expand the clinical program in Allergy/Immunology at Stanford. The proposed research project is already underway, and involves the examination of human CD4+ T cell subsets with restricted cytokine profiles. The division of CD4+ T cells into subsets is of fundamental importance in the regulation of isotype specific responses and in the regulation of responses to different types of antigens. Activation of CD4+ T cell subsets with inappropriate cytokine profiles may result in the development of allergic or autoimmune disease, or susceptibility to specific types of infection. The existence of CD4+ T cell subsets, though well established in mice, has not been well studied in man. The purpose of this proposal is to: 1. define the subtypes of human CD4+ T cell clones, and to determine under what conditions each subtype is preferentially stimulated. 2. examine how these subtypes of T cell clones regulate isotype specific responses in B cells. 3. determine how CD4+ T cell subsets may be involved in the pathogenesis of allergic disease. To study these problems, the candidate has developed exceptional culture techniques for generating and studying CD4+ clones. In addition, he has enlisted the support of other investigators to help apply molecular biology techniques to the analysis of his clones, and to provide a large number of valuable reagents necessary to evaluate the cellular interactions involved in the regulation of cytokine synthesis.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Academic/Teacher Award (ATA) (K07)
Project #
Application #
Study Section
Allergy & Clinical Immunology-1 (AITC)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Stanford University
Schools of Medicine
United States
Zip Code
Benjaponpitak, S; Oro, A; Maguire, P et al. (1999) The kinetics of change in cytokine production by CD4 T cells during conventional allergen immunotherapy. J Allergy Clin Immunol 103:468-75
DeKruyff, R H; Fang, Y; Umetsu, D T (1998) Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production. J Immunol 160:2231-7
Blotta, M H; DeKruyff, R H; Umetsu, D T (1997) Corticosteroids inhibit IL-12 production in human monocytes and enhance their capacity to induce IL-4 synthesis in CD4+ lymphocytes. J Immunol 158:5589-95
Lee, P P; Zeng, D; McCaulay, A E et al. (1997) T helper 2-dominant antilymphoma immune response is associated with fatal outcome. Blood 90:1611-7
Kim, T S; DeKruyff, R H; Rupper, R et al. (1997) An ovalbumin-IL-12 fusion protein is more effective than ovalbumin plus free recombinant IL-12 in inducing a T helper cell type 1-dominated immune response and inhibiting antigen-specific IgE production. J Immunol 158:4137-44
DeKruyff, R H; Gieni, R S; Umetsu, D T (1997) Antigen-driven but not lipopolysaccharide-driven IL-12 production in macrophages requires triggering of CD40. J Immunol 158:359-66
Oro, A S; Guarino, T J; Driver, R et al. (1996) Regulation of disease susceptibility: decreased prevalence of IgE-mediated allergic disease in patients with multiple sclerosis. J Allergy Clin Immunol 97:1402-8
Blotta, M H; Marshall, J D; DeKruyff, R H et al. (1996) Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis. J Immunol 156:3133-40
Secrist, H; Levy, S; DeKruyff, R H et al. (1996) Ligation of TAPA-1 (CD81) or major histocompatibility complex class II in co-cultures of human B and T lymphocytes enhances interleukin-4 synthesis by antigen-specific CD4+ T cells. Eur J Immunol 26:1435-42
Gieni, R S; Fang, Y; Trinchieri, G et al. (1996) Differential production of IL-12 in BALB/c and DBA/2 mice controls IL-4 versus IFN-gamma synthesis in primed CD4 lymphocytes. Int Immunol 8:1511-20

Showing the most recent 10 out of 22 publications