Abstract

Monoclonal and polyclonal antibodies to choline acetyl transferase (ChAT) will be used to immunoisolate messenger RNA which codes for the ChAT enzyme. Human placental tissue, which has been shown to be a rich source of ChAT, will be used as a source of polysomes for this effort. Human tissue (obtained by autopsies of Alzheimer's disease patients as well as controls who died of other causes) from the basal forebrain regions will be utilized to isolated mRNA so that disease specific and region specific libraries of complementary DNA can be created. These libraries will be used to screen for cDNA clones whose presence or absence is associated with Alzheimer's disease. The ChAT clones and the other Alzheimer related clones will be used to study the growth and development of brain cells in culture via in situ hybridization and RNA quantitation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Academic/Teacher Award (ATA) (K07)
Project #
5K07NS000965-04
Application #
3078397
Study Section
Neurological Disorders Program Project Review B Committee (NSPB)
Project Start
1985-04-01
Project End
1990-03-31
Budget Start
1988-04-01
Budget End
1989-03-31
Support Year
4
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Chicago
Department
Type
Schools of Medicine
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637