The proposed research will develop a system for genetic manipulation of Bacteroides and map key metabolic and antibiotic resistance loci on the chromosome of B. fragilis. Bacteroides are the most numerous bacteria in the normal human colon as well as the major pathogens in anaerobic infections. This study will characterize the conjugation system of pBF4, a self-transmissible drug resistance plasmid from B. fragilis. The pBF4 transfer system will be integrated into the Bacteroides chromosome, generating Hfr-like strains that transfer chromosomal loci. Nutritional auxotrophs and drug resistant strains will be used to generate a genetic map of the Bacteroides chromosome, providing important information about the transfer mechanism of anaerobic resistance plasmids and delineating the chromosomal organization of genes involved in key metabolic and drug resistance functions.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08AI000883-02
Application #
3078817
Study Section
Microbiology and Infectious Diseases Research Committee (MID)
Project Start
1988-07-01
Project End
1991-06-30
Budget Start
1989-07-01
Budget End
1990-06-30
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Type
Schools of Medicine
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093