The long term goal of this proposal is to provide Dr. Ana Karina Bedran-Russo, DOS, MS, PhD with the opportunity to develop into a well prepared, competitive and independent young scientist that will combine an active research career with teaching and practice. The applicant's ultimate goal is to improve patient care by contributing to translational research. Additional training in tissue engineering, oral biology and biomaterials obtained during this K08 Mentored Clinician Scientist Award will provide the necessary skills for a dental academic career track within a supportive multidisciplinary environment for the rapid transition of Dr. Bedran- Russo to an independent clinician scientist. Her research and training will be conducted under the guidance of Dr. James Drummond and Dr. Anne George. The applicant will build upon this research to seek additional funding. The research component of this grant aims to investigate the contribution of biochemical modifications to collagen on the properties of dentin and consequently on the durability of adhesive restorative dental treatments. A composite resin restoration last an average of 7 years in posterior teeth at which time failure at the interface is the primary reason given for replacement and accounts for 30-60% of all replaced restorations. The integrity of fibrillar type I collagen is an important issue when bonding to dentin since it is the main tooth component involved during restorative procedures. The hypothesis to be tested is that the artificially formed collagen cross-links in dentin will result in restoration interfaces with improved mechanical properties to withstand degradation over time.
The specific aims proposed are:
Aim 1. Determine the biochemical properties of dentin following treatment with different crosslinking agents. Biochemical properties (collagen cross-linking analysis - HPLC and immunohistochemical analysis) will be assessed in sound and caries-affected human dentin in both short and long term evaluations.
Aim 2 : Assess the mechanical properties of the treated dentin (sound and caries-affected) at short-term and long-term evaluations. Stiffness and hardness will be evaluated in specific sites using an atomic force microscope. Ultimate strength of dentin and bond strength of the dentin/restoration interface will be assessed using a universal testing machine. These combined biochemical and mechanical approaches will provide insights to correlate quality of dentin collagen and the long-term durability of restorations.

National Institute of Health (NIH)
National Institute of Dental & Craniofacial Research (NIDCR)
Clinical Investigator Award (CIA) (K08)
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Special Emphasis Panel (ZDE1-SS (55))
Program Officer
Hardwick, Kevin S
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University of Illinois at Chicago
Schools of Dentistry
United States
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Vidal, Cristina M P; Zhu, Weiying; Manohar, Suresh et al. (2016) Collagen-collagen interactions mediated by plant-derived proanthocyanidins: A spectroscopic and atomic force microscopy study. Acta Biomater 41:110-8
Leme, Ariene A; Vidal, Cristina M P; Hassan, Lina Saleh et al. (2015) Potential role of surface wettability on the long-term stability of dentin bonds after surface biomodification. J Biomech 48:2067-71
Silva, Ana Paula Pereira da; Gonçalves, Rafael Simões; Borges, Ana Flávia Sanches et al. (2015) Effectiveness of plant-derived proanthocyanidins on demineralization on enamel and dentin under artificial cariogenic challenge. J Appl Oral Sci 23:302-9
Padovano, J D; Ravindran, S; Snee, P T et al. (2015) DMP1-derived peptides promote remineralization of human dentin. J Dent Res 94:608-14
Bedran-Russo, Ana K; Pauli, Guido F; Chen, Shao-Nong et al. (2014) Dentin biomodification: strategies, renewable resources and clinical applications. Dent Mater 30:62-76
Castellan, Carina S; Bedran-Russo, Ana K; Antunes, Alberto et al. (2013) Effect of dentin biomodification using naturally derived collagen cross-linkers: one-year bond strength study. Int J Dent 2013:918010
Bedran-Russo, Ana K; Karol, Sachin; Pashley, David H et al. (2013) Site specific properties of carious dentin matrices biomodified with collagen cross-linkers. Am J Dent 26:244-8
Bedran-Russo, Ana K; Ravindran, Sriram; George, Anne (2013) Imaging analysis of early DMP1 mediated dentine remineralization. Arch Oral Biol 58:254-60
Dos Santos, Paulo Henrique; Karol, Sachin; Bedran-Russo, Ana Karina (2011) Long-term nano-mechanical properties of biomodified dentin-resin interface components. J Biomech 44:1691-4
Castellan, Carina Strano; Bedran-Russo, Ana Karina; Karol, Sachin et al. (2011) Long-term stability of dentin matrix following treatment with various natural collagen cross-linkers. J Mech Behav Biomed Mater 4:1343-50

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