We will investigate the role of carbonic anhydrase (CA) in the regulation and maturation of urinary acidification and its adaptation to metabolic and respiratory acidosis. First, in cultured cells obtained from inner medullary collecting ducts (IMCD) of either adult or immature rats, we will test the effect of acid loading on the rates of H+ pumping activity and of Cl-/ HCO3 exchange, on carbonic anhydrase (CA) activity and CA messenger RNA (mRNA). Second, we will test in rabbits the response to acid loading of CA activity in cortex, outer medulla and inner medulla and compare that activity to levels of mRNA for CA, band 3 and H+ ATPase. These experiments will reconcile changes in acid-base transport in the kidney with those in mRNA levels. We will compare these responses in vitro and in vivo in immature animals with those in adult animals. These studies will help us to understand why low birth weight infants are more susceptible to acidosis than are adults.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Clinical Investigator Award (CIA) (K08)
Project #
1K08DK001984-01
Application #
3080896
Study Section
Diabetes, Endocrinology and Metabolic Diseases B Subcommittee (DDK)
Project Start
1990-07-01
Project End
1995-06-30
Budget Start
1990-07-01
Budget End
1991-06-30
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461
Rosen, O; Suarez, C; Schuster, V L et al. (2001) Expression of carbonic anhydrase IV in mouse placenta. Am J Physiol Regul Integr Comp Physiol 280:R365-75
Brion, L P; Cammer, W; Satlin, L M et al. (1997) Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice. Am J Physiol 273:F234-45
Brion, L P; Zavilowitz, B J; Suarez, C et al. (1994) Metabolic acidosis stimulates carbonic anhydrase activity in rabbit proximal tubule and medullary collecting duct. Am J Physiol 266:F185-95
Matsumoto, T; Winkler, C A; Brion, L P et al. (1994) Expression of acid-base-related proteins in mesonephric kidney of the rabbit. Am J Physiol 267:F987-97
Brion, L P; Suarez, C; Zhang, H et al. (1994) Up-regulation of carbonic anhydrase isozyme IV in CNS myelin of mice genetically deficient in carbonic anhydrase II. J Neurochem 63:360-6
Brion, L P; Zavilowitz, B J; Rosen, O et al. (1991) Changes in soluble carbonic anhydrase activity in response to maturation and NH4Cl loading in the rabbit. Am J Physiol 261:R1204-13