This is an application for the Mentored Clinical Scientist Development Award to train Dr. Sandeep Jain. Its main educational objective is to provide Dr. Jain with the structured program necessary for him to become an independent biomedical scientist in the area of ocular neurobiology.
The specific aims of the proposed educational program consist of Dr. Jain's active participation in 1) didactic graduate course work in molecular biology and neuroscience at the University of Illinois at Chicago, 2) weekly ocular cell and molecular biology seminar and conferences at the Lions of Illinois Eye Research Institute (LIERI) and other medical affiliates of the University of Illinois at Chicago, and 3) a rigorous basic science research project under the supervision of a mentoring committee comprising four established scientists and clinicians. The mentoring committee comprises role models who will provide the applicant with a supportive setting conducive to learning, an ethos and commitment to excellence as well as accountability. The long term objective of the research proposal is to help delineate some of the cornea-resident cellular (keratocyte) and molecular (EphB1/ephrinB1) nerve guidance cues that play a role in the proper growth and patterning of axons during nerve regeneration in the cornea. We propose a novel hypothesis that cornea resident keratocytes play a hitherto unrecognized role of guiding axons during regeneration of corneal nerves.
The specific aims of the project are: 1) To correlate the pattern and course of EphB1/ephrinB1 expression with keratocyte activation and nerve regeneration, 2) To determine the effect of keratocyte ephrinBI ligand induced forward signaling on corneal nerve regeneration, and 3) To determine the effect of keratocyte EphB1 receptor induced forward signaling on nerve regeneration.

National Institute of Health (NIH)
National Eye Institute (NEI)
Clinical Investigator Award (CIA) (K08)
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Special Emphasis Panel (ZEY1-VSN (01))
Program Officer
Shen, Grace L
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University of Illinois at Chicago
Schools of Medicine
United States
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Shaheen, Brittany Simmons; Bakir, May; Jain, Sandeep (2014) Corneal nerves in health and disease. Surv Ophthalmol 59:263-85
Tibrewal, Sapna; Sarkar, Joy; Jassim, Sarmad H et al. (2013) Tear fluid extracellular DNA: diagnostic and therapeutic implications in dry eye disease. Invest Ophthalmol Vis Sci 54:8051-61
Sarkar, Joy; Chaudhary, Shweta; Jassim, Sarmad H et al. (2013) CD11b+GR1+ myeloid cells secrete NGF and promote trigeminal ganglion neurite growth: implications for corneal nerve regeneration. Invest Ophthalmol Vis Sci 54:5920-36
Hallak, Joelle A; Jassim, Sarmad; Khanolkar, Vishakha et al. (2013) Symptom burden of patients with dry eye disease: a four domain analysis. PLoS One 8:e82805
Namavari, Abed; Chaudhary, Shweta; Ozturk, Okan et al. (2012) Semaphorin 7a links nerve regeneration and inflammation in the cornea. Invest Ophthalmol Vis Sci 53:4575-85
Chaudhary, Shweta; Namavari, Abed; Yco, Lisette et al. (2012) Neurotrophins and nerve regeneration-associated genes are expressed in the cornea after lamellar flap surgery. Cornea 31:1460-7
Jain, Sandeep; De la Cruz, Jose; Kang, Eunkyo et al. (2012) Expression of phosphorylated heat shock protein 27 during corneal epithelial wound healing. Cornea 31:820-7
Namavari, Abed; Chaudhary, Shweta; Chang, Jin-Hong et al. (2012) Cyclosporine immunomodulation retards regeneration of surgically transected corneal nerves. Invest Ophthalmol Vis Sci 53:732-40
Sonawane, Snehal; Khanolkar, Vishakha; Namavari, Abed et al. (2012) Ocular surface extracellular DNA and nuclease activity imbalance: a new paradigm for inflammation in dry eye disease. Invest Ophthalmol Vis Sci 53:8253-63
Sarkar, Joy; Chaudhary, Shweta; Namavari, Abed et al. (2012) Corneal neurotoxicity due to topical benzalkonium chloride. Invest Ophthalmol Vis Sci 53:1792-802

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