The candidate is pursuing a career in academic surgery as an Assistant Professor of Surgery at the University of Nebraska Medical Center. His clinical interests include minimally invasive and robotic surgery and surgery of the abdominal wall His basic science interests include the regulation of wound healing, granulation tissue regression, and tissue regeneration. He has completed a 6 year residency in general surgery (Medical College of Wisconsin), a 1 year fellowship in minimally invasive surgery (MCW), and a 3 year fellowship in basic research (wound healing) with Fred Giinnell at the University of Texas Southwestern Medical Center. His short-term goal is to establish, with the help of extramural funding, a career in academic surgery at UNMC. His long-term goals include a greater understanding of the healing mechanism, progress towards achieving tissue regeneration, and advancement of minimally invasive and robotic surgery. The UNMC Surgery Chairman hired the candidate with the intent that the candidate would concentrate 75 percent of his efforts on research and career development. The candidate?s career development plan involves a combination of basic science research, didactic lectures, seminars, specialty courses, scientific meetings, research presentations, and scholarly debate/discussions. The candidate has 3 sponsors: a senior academic surgeon, a surgeon who is an established wound healing investigator, and a cell biologist experienced in apoptotic signal transduction. The latter two sponsors have NIH-funded laboratories. The candidate foresees a 4 year period to perform the proposed experiments and prepare an R01 application. The candidate's research plan will investigate the regulation of fibroblast survival in the collagen matrix. The clinical relevance is that since the fibroblast is the primary cell involved with complications/untoward effects of healing and scarring, control over fibroblast survival during healing should allow the clinician to minimize the negative effects of scarring in select clinical circumstances, such as in burn wound contracture, GI anastomotic stricture, or cirrhosis. The candidate has hypothesized that matrix anchorage upregulates focal adhesion kinase activity, which in turn upregulates the PI3K/Akt survival pathway and inhibits the tumor suppressor p53, ultimately promoting cell survival. Loss of matrix anchorage is hypothesized to reverse these effects and induce apoptosis. The involvement of each protein will be probed with mutated isoforms.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08GM000703-02
Application #
6525384
Study Section
Surgery, Anesthesiology and Trauma Study Section (SAT)
Program Officer
Somers, Scott D
Project Start
2001-09-30
Project End
2006-09-29
Budget Start
2002-09-30
Budget End
2003-09-29
Support Year
2
Fiscal Year
2002
Total Cost
$103,900
Indirect Cost
Name
University of Nebraska Medical Center
Department
Surgery
Type
Schools of Medicine
DUNS #
City
Omaha
State
NE
Country
United States
Zip Code
68198
Chao, Jie; Dai, Xiaoniu; Peña, Tiffany et al. (2015) MCPIP1 Regulates Fibroblast Migration in 3-D Collagen Matrices Downstream of MAP Kinases and NF-?B. J Invest Dermatol 135:2944-2954
Carlson, Mark A; Chakkalakal, Dennis (2011) Tensile properties of the murine ventral vertical midline incision. PLoS One 6:e24212
Frantzides, Constantine T; Madan, Atul K; Carlson, Mark A et al. (2009) Laparoscopic revision of failed fundoplication and hiatal herniorraphy. J Laparoendosc Adv Surg Tech A 19:135-9
Carlson, M A; Frantzides, C T; Shostrom, V K et al. (2008) Minimally invasive ventral herniorrhaphy: an analysis of 6,266 published cases. Hernia 12:9-22
Carlson, Mark A; Prall, Amy K; Gums, Jeremiah J (2007) RNA interference in human foreskin fibroblasts within the three-dimensional collagen matrix. Mol Cell Biochem 306:123-32
Carlson, Mark A (2006) Technical note: assay of cell quantity in the fibroblast-populated collagen matrix with a tetrazolium reagent. Eur Cell Mater 12:44-8
Eichler, Mark J; Carlson, Mark A (2006) Modeling dermal granulation tissue with the linear fibroblast-populated collagen matrix: a comparison with the round matrix model. J Dermatol Sci 41:97-108
Carlson, Mark A; Longaker, Michael T; Thompson, Jon S (2004) Modulation of FAK, Akt, and p53 by stress release of the fibroblast-populated collagen matrix. J Surg Res 120:171-7
Carlson, Mark A; Thompson, Jon S (2004) Wound splinting modulates granulation tissue proliferation. Matrix Biol 23:243-50
Frantzides, C T; Carlson, M A; Zografakis, J G et al. (2004) Minimally invasive incisional herniorrhaphy: a review of 208 cases. Surg Endosc 18:1488-91

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