Immune mediated platelet destruction occurs in idiopathic (immune) thrombocytopenia purpura (ITP), post transition purpura (PTP), neonatal isoimmune thrombocytopenia (NITP), and in other disease states. The antigens and antibodies involved in these conditions are not well characterized. Using techniques we have developed for identifying antigen bearing proteins of platelets, we will study the nature and structure of these platelet antigens and the antibodies which mediate platelet destruction. The technique involves electrophoretic separation of platelet membrane proteins, transfer to nitrocellulose paper, exposure to antibody, and detection of antibody binding using a radiolabeled second antibody. We propose to further characterize the PLA1 platelet antigen and to study the nature of the anti PLA1 antibodies in NITP. We will attempt to identify the platelet Fc receptor or Fab binding site to which platelet associated IgG may bind. Finally, we will further characterize the antigens and antibodies we have identified in a subject of children with ITP.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08HL001485-03
Application #
3081839
Study Section
(SRC)
Project Start
1983-07-01
Project End
1988-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Children's Hospital Boston
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
Mayer, J L; Beardsley, D S (1996) Varicella-associated thrombocytopenia: autoantibodies against platelet surface glycoprotein V. Pediatr Res 40:615-9
Beardsley, D S; Kauzmann, W J (1996) Local densities orthogonal to beta-sheet amide planes: patterns of packing in globular proteins. Proc Natl Acad Sci U S A 93:4448-53