Dr. Narula is in his third year as a fellow in the Pediatric Pulmonary Division at Columbia. He is fully trained and board certified in Pediatrics and is completing clinical training in Pediatric Pulmonology. His research training during the fellowship was initially focused on epithelial cell physiology (transepithelial fluid movement) t Columbia and he is now beginning work on epithelial cell biology (surface distribution of proteins in polarized epithelial cells) in Dr. Rodriguez-Boulan's laboratory at Cornell. The goal of the research plan is to elucidate the nature of the signals involved in the targeting of proteins into different surface domains of epithelial cells and the mechanism by which these signals operate. the human EGF receptor, NGF receptor, and several mutants of each of these will be transfected into MDCK cells and their surface distribution will be studied by biotinylation and targeting assays developed in Dr. Rodriguez-Boulan's laboratory. Basolateral sorting receptors will be identified by in vitro assays with perforated/transfected cells and then isolated by crosslinking experiments. The skills developed from these studies will enable Dr. Narula to study trafficking and polarization of CFTR in polarized epithelial cell lines expressing CFTR constitutively or in cells transfected with normal and mutant CFTR cDNA. Dr. Rodriguez-Boulan's laboratory is recognized for excellent training of post doctoral fellows and pioneering research on membrane and epithelial cells biology. In parallel with the research, Dr. Narula will take advanced courses in cell and molecular biology. Clinical activity to maintain skills will be limited to 15% effort and will be carried out in the Pediatric Pulmonary Division at Columbia where Dr. Narula will have an appointment as an Assistant Professor. On completion of the award, Dr. Narula will continue his academic career as an independent investigator in this Division.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
1K08HL002791-01
Application #
3083213
Study Section
Research Training Review Committee (RTR)
Project Start
1992-07-01
Project End
1994-06-30
Budget Start
1992-07-01
Budget End
1994-06-30
Support Year
1
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Type
Schools of Medicine
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027
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Narula, P; Xu, J; Kazzaz, J A et al. (1998) Synergistic cytotoxicity from nitric oxide and hyperoxia in cultured lung cells. Am J Physiol 274:L411-6
Spooner, P J; Small, D M (1987) Effect of free cholesterol on incorporation of triolein in phospholipid bilayers. Biochemistry 26:5820-5