The research plan proposes the characterization of intracellular mechanisms by which the cloned thrombin receptor mediates multiple cellular activities. Specifically, the candidate will: 1) identify G proteins which mediate thrombin signaling; 2) determine the functional sites for receptor-G protein interaction; and 3) define the cloned thrombin receptor's role in thrombin-induced activation of intracellular kinase cascades. These studies will allow the candidate to develop expertise in methodologies used to interrogate receptor-mediated signal transduction including: receptor expression in somatic cell and oocyte systems; chimeric receptor engineering; G protein binding assays; MAPK, Raf, and Ras activity assays; and the use of dominant negative mutants to explore the function of various signaling elements.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08HL003228-04
Application #
2734935
Study Section
Research Training Review Committee (RTR)
Project Start
1995-07-01
Project End
2000-06-30
Budget Start
1998-07-01
Budget End
1999-06-30
Support Year
4
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143