The broad objective of my research is to understand the molecular mechanisms of smooth muscle cell growth and to develop strategies to limit cell proliferation in arteries after injury based upon pathophysiology. The major limitations to therapeutic interventional procedures within peripheral or coronary arteries are thrombosis and intimal formation. We hypothesize that intimal cell growth and thrombosis are regulated by cell cycle proteins and prothrombotic proteins, respectively. Cytotoxic and cytostatic approaches may limit cell growth following vascular injury. In order to address these hypotheses, we propose three specific aims: (1) to optimize nonviral vectors to improve gene expression in vascular cells, (2) to examine the pathophysiology of smooth muscle cell growth in injured porcine and hyperlipidemic rabbit arteries and to develop cytotoxic approaches to limit intimal formation, and (3) to examine the role of thrombosis in intimal formation following vascular injury by investigating a factor X gene and a gene that encodes a factor Xa inhibitor.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08HL003473-06
Application #
6182660
Study Section
Research Training Review Committee (RTR)
Project Start
1996-06-01
Project End
2001-05-31
Budget Start
2000-06-01
Budget End
2001-05-31
Support Year
6
Fiscal Year
2000
Total Cost
$107,244
Indirect Cost
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
006471700
City
Rochester
State
MN
Country
United States
Zip Code
55905