Two cDNA libraries containing genes expressed specifically in rat hippocampus and rat cerebral cortex will be generated. Individual clones will be analyzed by Northern blotting and selected clones will be sequenced. This will yield statistical information on the genetic relationship between these evolutionary and phylogenetically contrasting brain structures. It will also provide the sequences of hippocampus and cortex-specific mRNAs. Using the genetic code, the protein sequence will be determined for each open reading frame. Synthetic peptides will be produced which match selected regions of each hippocampus or cerebral cortex-specific protein and these will be used to immunize rabbits. The resulting antipeptide antisera will be used in a number of immunocytochemistry experiments to probe brain slices using light and electron microscopy. This will provide anatomic information and suggest a functional role for each novel region-specific protein. Tissues to be probed include adult rat brain, developing rat brain, and human brain from Alzheimer's disease patients, schizophrenics and temporal lobe epileptics.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08NS000918-02
Application #
3083577
Study Section
Neurological Disorders Program Project Review B Committee (NSPB)
Project Start
1985-08-01
Project End
1990-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
City
San Diego
State
CA
Country
United States
Zip Code
92037