Previous studies in humans and experimental animals have provided evidence that antigen-specific T cells play a regulatory role in periodontal disease. However, little is still known regarding the properties to the T cell subpopulation(s) involved in regulating oral health or inflammation and periodontal bone loss, i.e., periodontal disease. The overall objective of the proposed reseach is to investigate T cell regulatory mechanisms which may be involved in local tissue inflammation and subsequent bone loss associated with periodontal disease. The studies will employ an experimental rat model and will specifically involve the isolation, generation and characterization of T cell clones which specifically help responses to the periodontopathogen Bacteroides gingivalis. The ability of these cloned T helper (Th) cells to regulate isotype-specific B cell growth and differentiation and to produce factors which mediate tissue destruction and repair will be evaluated. In addition, the influence of antigen-presenting cells in mediating T cell effects, and the ability of these cloned Th cells to influence periodontal bone loss in an experimental rat model will be determined.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Physician Scientist Award (K11)
Project #
1K11DE000232-01
Application #
3086145
Study Section
NIDR Special Grants Review Committee (DSR)
Project Start
1988-07-01
Project End
1993-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
1
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Alabama Birmingham
Department
Type
School of Medicine & Dentistry
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Katz, J; Russell, M W; Harmon, C C et al. (1995) Induction of salivary IgA responses to Streptococcus mutans antigen I/II after intranasal immunization. Adv Exp Med Biol 371B:1153-6
Katz, J; Harmon, C C; Buckner, G P et al. (1993) Protective salivary immunoglobulin A responses against Streptococcus mutans infection after intranasal immunization with S. mutans antigen I/II coupled to the B subunit of cholera toxin. Infect Immun 61:1964-71
Michalek, S M; Childers, N K; Katz, J et al. (1992) Liposomes and conjugate vaccines for antigen delivery and induction of mucosal immune responses. Adv Exp Med Biol 327:191-8
Katz, J; Leary, R M; Ward, D C et al. (1992) Humoral response to Porphyromonas (Bacteroides) gingivalis in rats: time course and T-cell dependence. Infect Immun 60:3579-85
Katz, J; Michalek, S M (1991) A method for generating antigen-specific rat T helper cell clones. J Immunol Methods 138:77-86
Katz, J; Michalek, S M; Beagley, K W et al. (1990) Characterization of rat T helper cell clones specific for Bacteroides gingivalis antigen. Infect Immun 58:2785-91