Abstract

The goal is to acquire a solid background in microbial and molecular genetics. The educational program will be carried out in the laboratory of Dr. Frederick Ausubel in the Department of Molecular Biology at the Massachusetts General Hospital, and will focus on characterization of the molecular genetic mechanisms in E. coli involved in adaptation to oxidant stress. The construction and characterization of a strain of E. coli carrying deletion for the manganese-superoxide dismutase gene (sodA), will be of initial interest. Laboratory work will be complemented by courses given in the MIT Graduate Biology Program, and by seminars at MGH, MIT and HMS. Phase II will take place under the guidance of Dr. Allan Walker, Chief of Pediatric Gastroenterology and Nutrition at MGH and CHMC, who has had a long term interest in studying the mammalian gut as an organ of host defense. Here, expertise gained in bacterial genetic manipulation during phase I will be used to develop a molecular genetic approach to the study of host/microbe interaction.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Physician Scientist Award (K11)
Project #
5K11DK001689-03
Application #
3086344
Study Section
(ADDK)
Project Start
1986-07-01
Project End
1991-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199