This MORE faculty development award is to provide Dr. Kale with training in molecular biology. Dr. Kale will spend five consecutive summers investigating the Drosophila ted gene, under the guidance and in the lab of Dr. Cavener. This gene function is needed for the adult fly to emerge from the pupal case. The approximate chromosomal position of ted is known. Genomic clones of this chromosomal region are available. To clone ted, the gene must be localized more precisely within this region. Dr. Kale will begin this by inducing ted mutations with X-rays, and then mapping the breakpoints of these lesions via Southern blotting. Once the position of ted gene has been narrowed down, Dr. Kale will identify potential ted transcription units, isolate cDNA clones for those RNAs, and sequence and map the ted gene. Finally, to prove that he has indeed cloned ted, Dr. Kale will insert ted genomic DNA into a germline transformation vector and determine whether the DNA can rescue ted mutations.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority School Faculty Development Awards (K14)
Project #
1K14GM000665-01
Application #
2020362
Study Section
Special Emphasis Panel (ZRG2-BIOL-1 (02))
Project Start
1997-05-01
Project End
2002-04-30
Budget Start
1997-05-01
Budget End
1998-04-30
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Alabama Agri and Mech University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
079121448
City
Normal
State
AL
Country
United States
Zip Code
35762
Garcia, Rebecca; Nguyen, Liem; Brazill, Derrick (2013) Dictyostelium discoideum SecG interprets cAMP-mediated chemotactic signals to influence actin organization. Cytoskeleton (Hoboken) 70:269-80