The research program will use a cell culture model to examine the response of bone cells to biomaterials. Cells will be cultured on corona- discharged-polystyrene or on dishes which have been sputter-coated with commercially pure titanium in order to assess whether exposure to Ti alters cellular response to growth factors. Three hypotheses will be tested: (1) Cell proliferation and matrix synthesis are altered when osteoblast-like cells are grown on Ti; (2) Matrix vesicle and matrix calcification are altered when these cells are cultured on Ti; and (3) Regulation of matrix synthesis and calcification by recombinant human transforming growth factor-beta1 (TGFbeta) are altered when cells are cultured by Ti. To test the validity of these hypotheses, the following aims are proposed: (1) To compare cellular proliferation and matrix synthesis of fetal rat calvarial (FRC) cells cultured on conventional corona-discharged-polystyrene (plastic) v. ti-coated surfaces; (2) To characterize matrix vesical structure and function and matrix calcification by FRC cells cultured on plastic v. Ti-coated surfaces; and (3) To determine whether culture on Ti alters FRC cell response to TGFbeta.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K15)
Project #
1K15DE000340-01A2
Application #
2128779
Study Section
NIDCR Special Grants Review Committee (DSR)
Project Start
1994-09-30
Project End
1999-09-29
Budget Start
1994-09-30
Budget End
1995-09-29
Support Year
1
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Texas Health Science Center San Antonio
Department
Orthopedics
Type
Schools of Medicine
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229